CCA - PROGRAMA DE PÓS-GRADUAÇÃO EM ZOOTECNIA (PPGZ)

CENTRO DE CIÊNCIAS AGRÁRIAS (CCA)

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Dissertations/Thesis


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2024
Description
  • WILSON ARAÚJO DA SILVA
  • ENZYME COMPLEXES ASSOCIATED OR NOT WITH Yucca schidigera EXTRACT FOR PIGLETS IN THE INITIAL STAGE
  • Advisor : LEONARDO AUGUSTO FONSECA PASCOAL
  • Date: Feb 29, 2024
  • Time: 15:00
  • Show Summary
  • Divided into four chapters, this thesis work was carried out to investigate the use of enzyme complexes associated or not with Yucca schidigera extract for piglets in the initial phase. In chapter 1, a systematic review was carried out on the addition of enzyme complexes to diets for piglets in the initial phase on productive performance and nutrient digestibility from a database composed of 76 diets, resulting from 40 studies. A search was carried out in electronic databases (Scopus, Web of Science and PubMed) through the CAPES Periodicals Portal collection, using a combination of words using the PICo method (Population, Interest and Context). The articles were exported to a reference management program and subjected to selection criteria, starting from a total of 5233 to 40 articles. It was observed that 52.5% of studies focus on the period from 2012 to 2022. China has the highest number of publications (9), followed by the USA (8) and Brazil (7). The enzymes most used in the complexes included in the diets were xylanase (33), β-glucanase (27), protease (16) and phytase (14). Single and increasing dosages of enzyme complexes improved performance and nutrient digestibility. However, an improvement in these parameters was not always observed, requiring further studies. In chapter 2, the biological effects of Yucca schidigera extract (YSE) on piglets were studied based on a literature review. The effects are associated with YSE saponins and polyphenolic compounds, related to improvements in performance, diet digestibility, intestinal morphology and gene expression, microbiota modulation and reduction of pollutants in excreta. More research is needed to identify the effectiveness of YSE supplementation for piglets. Chapter 3 was composed of a scientific article, in which the use of enzyme complexes on the digestibility of all-vegetable diets for piglets in the initial phase and excretion of pollutants was investigated. Twenty-four 35-day-old castrated male piglets were used, distributed in randomized blocks in three treatments: DC: Control diet; DCE1: DC with 200 g ton-1 of enzyme complex (Alltech® Allzyme SSF e+C: 700 HUT g-1 of protease, 300 SPU g-1 of phytase and 40 CMCU g-1 of cellulase); DCE2: DC with 400 g ton-1 of enzyme complex (Alltech® Vegpro® : 7500 HUT g-1 of protease and 45 CMCE g-1 of cellulase). DCE2 increased mineral availability, NDF digestibility and ADF. Enzyme supplementation increased the availability of P, Mg, Cu, Zn and Fe. There was a decrease in the excretions of P, Mg, Cu, Zn and a tendency towards lower Fe excretion with the use of enzyme complexes. Enzyme complexes with protease (140.0 to 2800.0 HUT Kg-1 of diet), phytase (60.0 SPU Kg-1 of diet) and cellulase (8.0 to 18.0 CMCU Kg-1 of diet) improve availability and decrease fecal excretion of minerals. Higher levels of protease (2800.0 HUT Kg-1 of diet) and cellulase (18.0 CMCU Kg-1 of diet) are necessary to improve the digestibility of NDF and ADF. In chapter 4, the objective was to evaluate the supplementation of enzyme complexes ssociated or not with plant extracts in diets for weaned piglets. Forty-eight 28-dayold animals were used, distributed in randomized blocks across six treatments: DC: Control Diet; DCY: DC with 125 g ton-1 of Yucca schidigera Extract (YSE) (De-Odorase®; guarantee level: 65 mg kg-1 of sapogenin); DCE: DC with 200 g ton-1 of enzyme complex (Alltech® Allzyme SSF e+C; guarantee levels: 700 HUT g-1 of protease, 300 SPU g-1 of phytase and 40 CMCU g-1 of cellulase); DCME: DC with 400 g ton-1 of multienzyme complex with emulsifier (Alltech® Allzyme Allsotution; guarantee levels: 640 AJDU g-1 of pectinase, 386 HUT g-1 of protease, 60 SPU g-1 of phytase, 32 BGU g-1 of β -glucanase, 16 XU g-1 of xylanase, 8 CMCU g-1 of cellulase, 4.5 FAU g-1 of amylase and 52 g kg-1 of polyethylene glycol glyceryl ricinoleate - PGRG); DCE+Y1: DCE + EYS (125 g ton-1 ); DCE+Y2: DCE + YSE (250 g ton-1 ). DCE+Y1 improved feed conversion. DCE, DCME and DCE+Y1 improved DM, MO, NDF, ADF, energy and P digestibility coefficients and reduced P excretion. Serum GGT levels increased with the addition of EYS, P decreased as a function of DCE +Y2 and AST decreased in DC-fed piglets. Greater jejunal AV/PC was observed with supplementations and increases in the thickness of the jejunal mucosa and absorptive area of piglets that consumed DCME. The number of goblet cells decreased when piglets consumed DCE+Y2. Higher liver glycogen levels were recorded for the groups that received DCE and DCE+Y2. Associations of exogenous enzymes with plant extracts decreased the relative expression of TNF-α mRNA; MUC-2 decreased with supplementation; that of NaPi-IIb increased with the use of DCE, while that of SGLT-1 increased with DCY and DCE; that of PEPT-1 increased with DCY, DCME, DCE+Y1 and DCE+Y2. Combinations of exogenous enzymes associated or not with plant extracts (125 g ton-1 of YSE and 52 g kg-1 of emulsifier) in diets for piglets optimize production efficiency, promote improvements in the digestibility and absorption of dietary nutrients, intestinal histology, and contribute to health intestinal and reduce P excretion. More research is needed on YSE inclusion levels for weaned piglets.
  • ANGÉLICA SOARES DE MENDONÇA LOPES
  • EFFECT OF THE ADDITION OF THE EXTRACT OF Poincianella pyramidalis (Tul.) IN THE DIET ON THE RUMINAL METABOLISM, PERFORMANCE AND QUALITY OF MEAT OF GOATS
  • Advisor : ARIOSVALDO NUNES DE MEDEIROS
  • Date: Feb 29, 2024
  • Time: 14:00
  • Show Summary
  • The objective of this research was to evaluate the effect of adding Catingueira extract (Poincianella pyramidalis Tul.) on intake, digestibility, performance, quantitative carcass characteristics and weight of commercial cuts of kids in confinement. To obtain the extract, leaves and branches of Catingueira (Poincianella pyramidalis Tul.) in full development stage were collected. The extract was prepared using an exhaustive maceration process for 72 hours using ethanol and, after concentration in a rotary evaporator, the extract was encapsulated in gelatin capsules. Thirty crossbred kids (Saanen x Alpino americano) were used, distributed in a completely randomized design (DIC), with 3 treatments and 10 replications. Treatments were formed according to plant extract doses (Poincianella pyramidalis Tul.), as follows: Treatment 1 – Standard diet + 0 mg of extract / kg of body weight (control); Treatment 2 – standard diet + 15 mg of extract / kg of body weight; Treatment 3 – standard diet + 30 mg of extract / kg of body weight. Intake, digestibility, performance, slaughter weight, hot and cold carcass weights, hot and cold carcass yields, weight loss on cooling and primary carcass cut weight were considered. There was no effect of adding Poincianella pyramidalis (Tul.) extract for any of the evaluated variables (P> 0.05). The average hot and cold carcass yield was 50.05 and 47.60%, respectively. Weight loss by medium cooling was 4.90% (p = 0.312). The doses of Poincianella pyramidalis (Tul.) extract used in the present study, for suckling kids, do not alter nutrient intake and digestibility, quantitative carcass characteristics and weights of primary carcass cuts.
  • MATEUS LACERDA PEREIRA LEMOS
  • CACTI POTENTIAL MODULATORY EFFECTS ON BACTERIAL COMMUNITIES IN DIFFERENT NICHES: INTESTINAL MICROBIOTA AND SPINELESS CACTUS SILAGE
  • Advisor : CELSO JOSE BRUNO DE OLIVEIRA
  • Date: Feb 29, 2024
  • Time: 14:00
  • Show Summary
  • The interest in the industrial exploration of cacti is emerging in the field of biotechnology due to recent research demonstrating their benefits in human and animal nutrition. The use of these plants not only fosters the development of the food, pharmaceutical, and livestock industries, particularly in the semi-arid regions, but also promotes the preservation of ecosystems and ethnocultural practices. With recent advances in microbiology and the discovery of the microbiota's importance, such as the influence of intestinal microbiota on its host, there arises the need to evaluate the effects of cacti consumption on the intestinal microbiota, which could potentially interfere with their health-promoting effects. Furthermore, the interest in using cacti for forage conservation through silage methods is exponentially increasing, especially for forage palm, whose cultivation is relevant in semi-arid climates or tropical countries, such as the northeastern region of Brazil. It is crucial to understand the unique fermentative processes that occur during palm fermentation. In this context, investing in research on lactic acid bacteria becomes essential, as they are the main intermediaries of fermentation, with applications ranging from the use of inoculants to probiotic strains. The first study aimed to investigate the effects of "xique-xique" juice (XQ) - Pilosocereus gounelli - on the intestinal microbiota of healthy and colitis-induced rats. The hypothesis was based on the anti-inflammatory, antioxidant, and potential prebiotic properties of XQ, suggesting that XQ could modulate the composition of the intestinal microbial community to improve health. The results indicated significant changes in intestinal microbial diversity in groups receiving XQ, associated with a reduction in key taxa related to intestinal inflammation. The XQ group showed a positive association with specific microbial families, such as Ruminococcaceae, Lachnospiraceae, Christensenellaceae, and Saccharimonadaceae. Additionally, a significant increase was observed in metabolic pathways associated with post-colitis intestinal recovery, such as short-chain fatty acid production and mucosal barrier function. The second study aimed to characterize the microbial dynamics during palm silage fermentation, revealing a pattern of rapid fermentation with pH drop within two weeks post-ensiling. Lactobacillus, Weissella, and Pediococcus were identified as the main microorganisms involved in palm fermentation, with Weissella being dominant both during the initial fermentative stage and the aerobic stability phase. Functional analyses demonstrated activity in the phosphoketolase pathway and other heterofermentative routes, highlighting palm silage as a suitable option for silage production in tropical countries. The third study focused on the genomic investigation of the functionality of Lactiplantibacillus plantarum CNPC024, a potential probiotic strain isolated from artisanal cheese. In silico analysis confirmed the bacterium's suitability for gastrointestinal conditions and safety, with the absence of mobile resistance genes, revealing clusters of genes associated with bacteriocin production, and CRISPR sequences. The study concluded that L. plantarum CNPC024 has favorable characteristics, such as growth in dairy products and the potential to modulate host immune responses, suggesting its potential as a probiotic candidate. These three scientific studies, addressing different aspects related to plants and microorganisms, contribute to understanding potential nutraceutical, food-related, and health-promoting applications in scientific research.
  • JOSÉ DANRLEY CAVALCANTE DOS SANTOS
  • THERMOREGULATION OF GOATS RAISED IN SEMIARID EQUATORIAL ENVIRONMENT
  • Advisor : EDILSON PAES SARAIVA
  • Date: Feb 29, 2024
  • Time: 09:00
  • Show Summary
  • Goats raised in production systems in the semiarid region are often challenged by high temperatures, low water availability, food scarcity, and high radiant heat loads experienced in the rearing environment. The aim of this study was to evaluate through remote sensing (biologgers) how native goats modulate their responses to maintain homeothermy in the face of semiarid challenges. For this purpose, twenty-six goats of Moxotó and Canindé breeds underwent surgical procedures, during which temperature sensors (Star Oddi, DST mili-T) were implanted to perform frequent measurements of subcutaneous and intraperitoneal temperatures. Air temperature, humidity, solar radiation, wind speed, rainfall, forage mass, soil temperature, and average radiant temperature were measured to characterize the challenges faced by the animals. Our results showed effectiveness in the continuous measurement of intraperitoneal and subcutaneous temperature through remote sensing. Subcutaneous temperature varied on average by 8°C, with peaks between 12:00 and 13:00 and lows between 04:00 and 06:00. The average intraperitoneal temperature was 39.0°C, with peaks between 14:00 and 15:00. The vasomotor index showed an average amplitude of 0.94°C, reaching a peak at 20:00. During the hottest days, Moxotó and Canindé goats registered peaks of body temperature of up to 41.1 and 41.3°C, respectively. Exposure to higher ambient temperatures resulted in a range in body temperature of up to 3.1°C. Native goats, when exposed to hotter days, even in conditions with water and food available, were unable to maintain their temperature within narrow limits. In conclusion, the use of remote sensing in long-term studies will allow advances in understanding the adjustments made by goats to maintain homeothermy in the face of a full set of stressors (high temperatures, high radiant heat loads, water, and food scarcity).
  • JOSÉ RAY MARTINS FARIAS
  • Saline concentration and microbiological profile of groundwater available for consumption by domestic ruminants in semi-arid agroecosystems in the state of Paraíba, Brazil
  • Advisor : ALBERICIO PEREIRA DE ANDRADE
  • Date: Feb 29, 2024
  • Time: 09:00
  • Show Summary
  • The scarcity of fresh water in semi-arid regions has driven the use of brackish groundwater to water ruminants. Therefore, knowing the physicochemical and microbiological characteristics of brackish groundwater is a fundamental step to support the breeding of domestic ruminants. The objective of this study was to evaluate the spatial and temporal variability of total solids levels (TSD) and the microbiological profile of brackish groundwater in semi-arid agroecosystems in the state of Paraíba, Northeast Brazil, analyzing their implications for the production of domestic ruminants. The microbiological profile and STD levels of the water available for consumption by ruminants was carried out in the Cariri Oeste and Seridó Oriental microregions, both located in the Messorregião da Borborema. Data collected during the years 2015, 2016 and 2017 from 25 artesian wells, 14 in the west of Cariri and 11 in the east of Seridó, were analyzed using descriptive and multivariate statistics. The study showed that the groundwater from wells in the two micro-regions has high levels of DST – total dissolved solids (735.9 to 14,459.9 mg/L), being characterized as fresh water (2.45% of wells), brackish (68.03 from the wells). And salty (29.50% of wells). The presence of total coliforms and Escherichia coli. was detected in 100% and 30% of the water from the sampled wells, respectively. The concentration of DST in the groundwater of Cariri Occidental and Seridó Oriental varies spatially, being classified as brackish or saline. Groundwater presents major limitations for the consumption of domestic ruminants, including the high concentration of salts and the presence of contaminating microorganisms.
  • DIEGO FRANCISCO OLIVEIRA COELHO
  • Effect of diets with high proportions of cactus on the intestinal microbiome and performance of confined sheep
  • Advisor : EDSON MAURO SANTOS
  • Date: Feb 29, 2024
  • Time: 08:00
  • Show Summary
  • This study comprised two experiments. First, a metagenomic analysis was carried out on the feces of sheep fed with cactus-based diets, divided into four treatments with levels of 7.5%, 15%, 30% and 45% inclusion of buffel grass hay. The objective was to observe the impacts on intestinal microorganisms and the incidence of diarrhea. Feces were collected weekly for fecal score analysis, in addition to metagenomic assessments on the 1st and 40th day. The phyla Bacteroidetes and Firmicutes predominated in all diets, while Spirochaetes and Verrucomicrobia were observed in diets with 30% and 45% hay inclusion. At the taxon level, Bacteroides and Rikenellaceae_RC9_gut_group were predominant in the phylum Bacteroidetes, and Oscillospirace_UCG-005 and members of Lachnospiraceae were the predominant taxa of Firmicutes. The fecal score varied between 0 and 2 for all diets, with no cases of diarrhea or abundance of pathogenic bacteria. There was greater abundance and lower taxonomic richness in samples from animals that ingested diets with 7.5% and 15% hay inclusion, and the opposite effect of other diets. In the second experiment, nutrient intake, performance and cost of diets fed to sheep fed diets containing different proportions of cactus and buffel grass hay were evaluated. The objective was to identify the optimal level of hay inclusion in cactus-based diets. With 30% inclusion, the animals obtained higher dry matter intake (DMI), higher average daily gain (ADG) and higher total gain (GT). The maximum point for most consumption variables was reached when the animals consumed a diet with 25% hay inclusion. Higher feed conversion (CA) and feed efficiency (EA) were observed in animals that consumed the diet with 45% hay inclusion. The economic evaluation showed that, as cactus was reduced in the diet, it became more expensive. However, the net income generated by the 30% hay inclusion diet made this diet the most advantageous. Diets based on forage cactus with 30% hay inclusion promote the formation of a beneficial intestinal microbiome for sheep, in addition to providing greater weight gains and income.
  • THALIS JOSE DE OLIVEIRA
  • DIETARY SUPPLEMENTATION OF GLUTAMINE AND GLUTAMIC ACID ON PERFORMANCE, GENE EXPRESSION AND INTESTINAL MORPHOLOGY IN EUROPEAN QUILTS WITH DELAYED ACCESS TO FOOD POST-HATCHING.
  • Advisor : PATRICIA EMILIA NAVES GIVISIEZ
  • Date: Feb 29, 2024
  • Time: 08:00
  • Show Summary
  • The study was developed with the aim of evaluating dietary supplementation of Glutamine (Gln) and Glutamic acid (Glu) on the performance, gene expression of transporters and intestinal morphology of European quails subjected to delayed access to post-hatch food. 360 one-day-old European quails were used, distributed in a completely randomized design and a 3 x 2 factorial scheme, with six treatments and five replications of 12 birds each. The treatments corresponded to three times of late access to food (0, 24 and 48 hours) and two levels of glutamine supplementation associated with glutamic acid (0% Gln+Glu and 0.2% Gln+Glu). Live weight and feed intake were evaluated weekly, and weight gain and feed conversion were calculated between 1 – 21 and 1 – 42 days of age. The gene expression of Sglt1, Glut2 and Snat1 and Snat7 was evaluated by qPCR at 7, 14 and 21 days of age in jejunum samples. Birds submitted to late access of 48 hours showed lower performance results compared to those fed earlier. Supplementation of 0.2% Gln+Glu in the diet did not influence the performance of the birds. There was an interaction between the factors late access and diet on the relative expression of Sglt1 and Glut2 mRNA at 7, 14 and 21 days of age. Birds submitted to delayed access of 24 and 48 hours and the diet with 0.2% Gln+Glu supplementation showed the highest levels of Sglt1 mRNA expression. Glut2 mRNA expression was higher at seven days of age in birds submitted to delayed access of 24 and 48 hours and a diet with 0.2% Gln+Glu. The results showed a significant interaction between access to food and diet in the expression of Snat1 and Snat7 mRNA at different ages in quails. Snat7 expression at 7 and 14 days and late access to 48-hour and supplemented food resulted in the highest expression, and at 21 days, there was a reduction in the groups with late access. Regarding Snat1 expression at 7 and 14 days, the group with delayed access of 24 and 48 hours and supplemented showed higher levels of Snat1 expression, while at 21 days there was a reduction. Supplementation with 0.2% Gln+Glu resulted in an increase in VA for both birds with immediate access and those with a 24-hour delay. The immediate access and 24-hour delay groups exhibited the highest VSA results, regardless of supplementation. In conclusion, regardless of supplementation, late access to food for 24 and 48 hours was detrimental to the performance of European quails up to 21 days of age. Glutamine and glutamic acid supplementation increased Sglt1 mRNA expression in groups that received food late. Late access did not influence Glut2 mRNA expression, but supplementation promoted an increase in Glut2 mRNA expression. Glutamine and glutamic acid supplementation increased Snat1 and Snat7 mRNA expression in groups with delayed access to food until 14 days of age. Supplementation with 0.2% Gln+Glu in the diet of European quails, when subjected to delayed access to food in the first 48 hours of life, alone is not sufficient to guarantee ideal performance and repair of the intestinal mucosa.
  • FRANCISCO ALDEVAN MIRANDA BEM
  • -
  • Advisor : LEONARDO AUGUSTO FONSECA PASCOAL
  • Date: Feb 28, 2024
  • Time: 08:30
  • Show Summary
  • This thesis work was composed of a literature review, systematic review and meta-analysis of the implications of neutral and acid detergent fiber in the diet of pregnant sows and on productive performance during pregnancy and lactation. In chapter I it is observed that several studies have been carried out in this field of investigation and, depending on the source and level of fiber used, its interaction with the intestinal microbiota has shown strong evidence of improvements to the production system, despite some inconsistencies when between individual studies. Chapters II and III consist of a systematic review and meta-analysis of the relationship between fiber in its fractions determined by NDF and FDA, on productive performance during pregnancy and lactation respectively. Systematized searches were carried out in three electronic databases (Pub Med, Web of Science and Scielo), which resulted in findings of 1934 articles. After exclusion and inclusion criteria, the database was composed of 67 studies, of which 32 had the same experimental period (the entire gestation phase) and performance data from 4538 sows were extracted for meta-analysis. Analysis of variance was performed using SAS (9.3) mixed model software using the PROC MIXED procedure and the RANDOM option where genetics, birth order, digestible lysine concentration and metabolizable energy were used as variation factors (random effect). For weight gain and bacon thickness during pregnancy, there was no difference (P<0.05) depending on the diet with low, medium and high fiber content based on NDF and ADF values. The number of piglets born and birth weight did not differ significantly (P<0.05). The duration of farrowing of sows fed diets with a medium fiber content (18.17 NDF and 7.07 ADF) was shorter, as was the number of stillbirths in the high fiber group (25.58 NDF and 14.01 of FDA). The body conditions of sows during lactation and the weaningestrus interval were not affected by the NDF and ADF content of the diets (p<0.05). Voluntary feed intake during lactation was improved with the use of fiber-enriched diets. The inclusion of dietary fiber in the feed of pregnant sows, in NDF and ADF values, increases the number of weaned piglets and the weight of piglets at weaning. Pregnancy diets with average values of 14.01% ADF and 25.58% NDF did not affect performance and provided productivity gains.
  • ALAN DOUGLAS DE LIMA ROCHA
  • Salmonella enterica in water bodies under agricultural impact in the State of Paraíba: diversity of serotypes and risk factors associated with contamination
  • Advisor : CELSO JOSE BRUNO DE OLIVEIRA
  • Date: Feb 28, 2024
  • Time: 08:00
  • Show Summary
  • Salmonella enterica, a widespread zoonotic foodborne pathogen, remains a significant public health concern and economic burden worldwide. It is estimated that Salmonella ssp. cause 93.8 million cases of gastroenteritis worldwide annually, leading to 59,100 deaths. In the United States (US), gastroenteritis caused by non-typhoid Salmonella alone is estimated to affect approximately one million people annually, resulting in approximately $3.7 billion in medical costs. Despite historic containment efforts focused on oral-fecal transmission and quality control in food production, the incidence of Salmonella persists, with concerning outbreaks associated with plant-based foods in recent years. An underestimation of Salmonella in aquatic environments and its potential long-term persistence raises concerns about the role of water in Salmonella epidemiology. Recent studies have shown that Salmonella's ability to remain viable in non-enteric environments is considerably greater than previously believed. Furthermore, Salmonella is not a microbial target for methods typically used to assess water quality. These aspects may point to the possibility that the presence and persistence of Salmonella in aquatic environments may be underestimated. This study investigated the environmental factors that influence Salmonella in natural watersheds used for agricultural and livestock purposes in Paraíba, Brazil. Furthermore, it carried out a survey of the main serovars involved in the contamination of these water resources.
  • ANNA NEUSA EDUARDA FERREIRA DE BRITO
  • SUPERDOSING PHYTASE FOR WHITE LAYERS
  • Advisor : FERNANDO GUILHERME PERAZZO COSTA
  • Date: Feb 28, 2024
  • Time: 08:00
  • Show Summary
  • The aim of this study was to determine the influence of phytase superdosing on the performance, internal and external quality of eggs and intestinal morphometry of white layers aged 40 to 80 weeks that received diets supplemented with phytase and on bone parameters of white layers 80 weeks. A total of 480 white layers distributed in six treatments with 10 repetitions and 8 white layers per repetition, namely: positive control (CP), negative control (CN), negative control with 600 FYT (CN-600FYT), negative control with 1200 FYT (CN-1200FYT), negative control with 1800 FYT (CN-1800FYT) and negative control with 2400 FYT (CN-2400FYT). Eight birds were slaughtered using electronarcosis treatment to collect the intestinal segments. A significant difference was observed for the diets supplemented with phytase in the variables feed consumption, egg production, egg mass, conversion per egg mass and per dozen, house weight, yolk weight and albumen weight. And it was observed that shell thickness was greater for the positive control and negative control diets. After slaughter, intestinal segments and tibias were collected. A greater villus-crypt ratio was observed for birds that received diets with phytase supplementation. There was a significant effect for mineral matter and breaking resistance for birds from CP (40.816 a) and CN-600FYT (27.314 a). It was concluded that the superdosing of phytase was efficient for the performance and intestinal health of light layers aged 40 to 80 weeks. And for layers at 80 weeks of age, the dosage of 600 FYT for light layers was effective for greater bone health in the birds.
  • MIKAELLE DE SOUSA DUTRA
  • NUTRITIONAL MONITORING OF RUMINANTS RAISED ON PASTURE USING NIR SPECTROSCOPY IN PORTABLE EQUIPMENT
  • Advisor : ROBERTO GERMANO COSTA
  • Date: Feb 28, 2024
  • Time: 08:00
  • Show Summary
  • Near Infrared Reflectance Spectroscopy (NIRS) has proven to be a promising tool for estimating the nutritional value of the diet of grazing cattle, making it possible to obtain representative information on food consumption and diet quality quickly and non-destructively. This study sought to develop calibration and validation models to estimate the content of Crude Protein (CP), In vitro dry matter digestibility (DIVMS), indigestible neutral detergent fiber (iNDF), Neutral detergent fiber (NDF), Detergent fiber acid (FDA) and dietary lignin using portable equipment. Samples of cultivated pasture from five Brazilian states were collected by simulated grazing, and spectra of cattle feces were obtained under three conditions: fresh, dried without grinding, and dried and ground, using the MicroNir OnSite-W device from VIAVI Solutions Inc. with reading range from 950 to 1650nm. The most effective mathematical pretreatments were SNV+SG1.4 and SNV+SG2.4, applicable to all types of sample processing, regardless of the period of the year. The calibration models for DIVMS presented higher R², maintaining consistency in different conditions (F=0.66; SSM=0.71; SM=0.74), indicating an equivalence of performance for application in samples devoid of moisture. However, the results indicate a limited ability of calibration models to accurately predict NDF, ADF and lignin contents in bovine fecal samples, regardless of processing. This highlights the complexity in analyzing these parameters and highlights the continuous need to improve analysis and 362 calibration techniques, as well as the importance of a robust database to improve the accuracy of estimates.
  • ANDERSON LOPES PEREIRA
  • Supplementation strategies for heifers on tropical pasture
  • Advisor : EDSON MAURO SANTOS
  • Date: Feb 27, 2024
  • Time: 14:00
  • Show Summary
  • Supplementing heifers on tropical pastures during the rearing phase requires nutritional strategies that make it possible to maximize productive performance and reproductive efficiency, even in unfavorable climatic conditions for forage production. Therefore, two studies were carried out with the objective of evaluating different supplementation strategies for primiparous heifers kept on tropical pasture in different seasons. In the first chapter, the objective was to evaluate the effect of supplementation during three periods (rain/dry, drought and drought/rain) on the productive and reproductive performance of Nelore heifers in tropical pasture. 28 Nelore heifers were used, approximately eight months old and with an average body weight of 205.80 kg ± 16.50 kg. The heifers were subjected to two supplementation strategies, the 16 CP strategy (supplement containing 16% crude protein (CP)) and the 20 CP strategy (supplement containing 20% CP). The experimental design used was completely randomized in a factorial arrangement, with two supplementation strategies and three periods (rain/dry, dry and dry/rain) with 14 replications (heifer). The experimental period consisted of three periods: rain/dry (56 days), drought (84 days) and drought/rain (60 days). For performance, the effect of the interaction between strategy x period was verified for the variables final body weight (FBW, kg) (P<0.032), total weight gain (TWG, kg) (P<0.039), stocking rate (UA .ha- 1) (P<0.020) and weight gain per area (GPA, @.ha- 1) (P<0.033). The lowest averages were observed during the dry period for the 20 CP strategy (271.74 kg; 31.48 kg; 3.99 AU.ha- 1 and 7.35 @.ha- 1, respectively). Only for FBW (P=0.012), TWG (P=0.007) and stocking rate (P=0.077) isolated treatment effects were observed, with the highest averages for the 16 CP strategy (337.89 kg; 131.52 kg; 4.14 AU.ha- 1). For period, an effect was verified for all performance variables (P<0.001). In the dry period, the lowest average for average daily gain (ADG, kg.day- 1) was observed, obtaining gains of 0.434 kg.day- 1 . In relation to the reproductive efficiency of heifers, the 16 CP strategy was superior to the 20 CP strategy , as it enabled a higher pregnancy rate of more than 80%. There was no effect of the interaction between strategy x period for any of the productive and morphological variables (P>0.05). An isolated effect of treatment (P<0.05) was verified for all variables, except leaf:stem ratio (P=0.485). The 16 CP strategy obtained higher averages compared to the 20 CP strategy for the productive and morphological variables. An effect of the period (P<0.001) was verified for all productive and morphological variables of the forage. During the dry/rainy period, the highest averages were observed for green leaf mass (GLM, kg.ha- 1 ), green blade mass (GBM, kg.ha- 1) and leaf:stalk ratio (1,750 kg.ha- 1 ; 0.907 kg.ha- 1 ; 1.40, respectively). In the chemical composition of the forage, only period effect was observed (P<0.001), where in the dry period the highest averages were obtained for dry matter (DM), neutral detergent fiber (NDF) and acid detergent fiber (ADF) ( g.kg-1) (514.90; 766.40; 447.30 g.kg- 1, respectively) and lower for crude protein (CP, g.kg-1 ) obtaining 52.80 g.kg-1 . The 16 CP strategy provided better performance of heifers on grazing, and the dry/rainy period proved to be more favorable due to the quantity and quality of forage, associated with supplementation. In the second study, the objective was to evaluate three protein-energy supplementation strategies on the performance and biochemical parameters of heifers kept on tropical pasture during the rainy season. 30 ringed heifers, approximately 10 months old and with an average body weight of 195.57 kg ± 15.89 kg, were used. The heifers were subjected to three supplementation strategies: Strategy 0.3% (offer of 0.3% of body weight - BW), Strategy 0.6% (offer of 0.6% of BW) and Strategy 0.9% (offer of 0.9% of BW), for 77 days, with the first three cycles lasting 21 days each, and the last cycle lasting 14 days. The design was completely randomized, with three supplementation strategies and five replications (paddock). An effect of the strategy x cycle interaction was verified for final body weight (FBW, kg) (P<0.001) and weight gain per area (GPA, @.ha- 1) (P<0.001). The highest average FBW was recorded in cycle 4 for the 0.9% strategy (266.11 kg), while the lowest GPA occurred in the 0.3% strategy (5.60 @.ha- 1) in the same cycle. Isolated effects of strategy were observed for dry matter intake (DMI, kg.day- 1 and % BW), FBW (P=0.018), total weight gain (TWG, kg) (P=0.009), average daily gain (ADG, kg.day- 1 ) (P=0.007) and GPA (P<0.001). The 0.6% and 0.9% strategies presented the highest averages for TWG (63.82 and 69.83 kg, respectively) and ADG (0.834 and 0.922 kg.day- 1, respectively). A cycle effect was verified for all performance variables analyzed (P<0.001). For DMI in kg.day- 1 and FBW, an increase was observed throughout the grazing cycles. Blood parameters showed effects of supplementation for alkaline phosphatase (ALP) and aspartate aminotransferase (ALT), with higher ALP means (290.31 and 266.10 U/L) for the 0.6% and 0.9% strategy, respectively, and higher average ALT (31.44 U/L) in the 0.3% strategy. No effects (P<0.05) of interaction between strategy x cycle or the isolated effect of strategy were observed for any of the grass's productive and morphological variables. However, there was a significant effect of the cycle (P<0.001) for all productive and morphological variables of the grass. The 0.6% strategy for heifers on tropical pasture favored productive performance, with greater weight gain and gain per area, without causing metabolic damage to the animals. Both studies highlighted the importance of supplementation strategies for heifers on tropical pastures, especially during challenging periods. Supplementation can significantly improve productive and reproductive performance, ensuring the health and adequate development of the herd. These findings are essential to guide nutritional management practices that aim to optimize livestock production in tropical regions.
  • DANILO DANTAS DA SILVA
  • Consortium of forage cactus and grasses: effects on the soil seed bank, soil macrofauna and nutritional value
  • Advisor : ARIOSVALDO NUNES DE MEDEIROS
  • Date: Feb 27, 2024
  • Time: 09:00
  • Show Summary
  • This thesis is divided into three chapters. In the first, the study evaluated the composition, dynamics and diversity of the soil seed bank in a toposequence, considering the presence of the palm consortium with grasses. Soil samples were collected in three altitudinal gradients: top (PT), mid-slope (PM) and slope (PE). For comparison, samples were collected in a paddock of native pasture, enriched with the same grasses as forage cactus cultivation. The floristics of the area with forage palm presented 36 species, belonging to 31 genera and 16 botanical families. The families Poaceae, Fabaceae and Malvaceae showed greater species richness. The native pasture, despite being located in an area adjacent to the top, presents differences in its floristic composition (24 species, 22 genera and 11 families). The toposequence seed bank presents high diversity and equity, which proves that the community is floristically heterogeneous and with few dominant species. The second study sought to analyze the influence of this consortium on the frequency of occurrence, richness and diversity of soil macrofauna. Provid-type traps were installed in three altitude gradients of the topossequence (top, mid-slope and slope) and in the native pasture during two periods (dry and rainy). A total of 18,376 individuals were collected (8,723 in the dry season and 9,653 in the rainy season), distributed in 21 orders (17 top, 18 mid-slope, 16 on the slope and 15 in the pasture). The taxonomic group Hymenoptera Formicidae was predominant in the areas. Richness was lower in the dry period and the orders Archaeognatha, Scorpiones and Polydesmida occur exclusively on the slope, while Scolopendrida, on the mid-slope. The Shannon index was higher on the slope in both periods, driven by the lower participation of the order Hymenoptera. The third study investigated how changes in soil carbon, nitrogen and phosphorus stocks along the toposequence impact the nutritional value of Nopalea cochenillifera (L.) Salm-Dyck. The results revealed a linear increase in P stocks with the altitudinal gradient, while soil C and N were higher at the top and on the slope. It was also observed that concentrations of crude protein and minerals (N, P, K, Ca, Mg and S) increased, while dry matter, total carbohydrates and fibrous compounds decreased at higher altitudes. These findings indicate variations in soil C, N and P stocks along the toposequence, with only P concentrations increasing with increasing altitude. The increase in soil P stocks is associated with a reduction in the concentration of fibrous compounds in N. cochenillifera cladodes.
  • LUANA DA SILVA CORDEIRO
  • AGRONOMIC RESPONSES AND BROMATOLOGICAL COMPOSITION OF FORAGE CACTUS IN RESPONSE TO SOIL FERTILITY LEVELS.
  • Date: Feb 27, 2024
  • Time: 08:00
  • Show Summary
  • The objective was to evaluate the effects of combined doses of nitrogen and potassium on cactus cultivation. The research was carried out in the countryside of Paraíba, in the municipality of Campina Grande, in the state of Paraíba, Brazil. The experimental design used was randomized blocks in split plots, with six replications. The plots were represented by the combined doses of nitrogen (N) and potassium (K) and the subplots by the application of lime (with and without lime). The spacing used was 1.70 m × 0.30 m × 0.10 m, which provided a density of 100,000 plants ha-¹. Five doses of N (7.8; 46.8; 78; 109 and 148 kg ha-¹) and five doses of K (30; 180; 300; 420 and 570 kg ha-¹) were combined. The variables were subjected to analysis of variance, F test and polynomial regression. The morphometric and productive variables showed quadratic behavior in relation to the combined doses. There was no significant difference in chemical composition. For the variable of plant height (AP), area related to primary cladode (ARC1), area related to total cladode (ARCT) and cladode area index (IAC) the combination between 109 kg of N and 180 kg of K, offered the highest averages, for the area variable related to the secondary cladode (ARC2), the dose of 46.6 kg of N with the same dose of K mentioned previously achieved greater area growth, for the dry matter content (MS ) and dry matter production (DMP), the combined doses of 7.8 kg of N and 30 kg of K, obtained greater responses. The subplots with limestone addition had a positive effect on all variables, except for green matter production (PMV), where 109 kg of N and 420 kg of K prevailed without limestone. The increase in combined doses of 109 and 180 kg ha-¹, N and K, respectively, when applied to the soil with the addition of limestone brings viability for palm nutrition.
  • HEMERSON JOHNSON XAVIER DE ASSIS
  • Carcass characteristics and meat quality of feedlot sheep fed diets based on BRS Capiaçu silage added with corn bran.
  • Advisor : PAULO SERGIO DE AZEVEDO
  • Date: Feb 26, 2024
  • Time: 14:00
  • Show Summary
  • The objective of this work was to evaluate the carcass characteristics and meat quality of confined sheep fed diets based on BRS Capiaçu silage in comparison with animals fed diets based on corn silage. We used 21 crossbreed animals, entire males, aged approximately 90 days and average live weight of 26.0kg, confined in individual stalls and distributed in an experimental design in randomized blocks, in which body weight (BW) was used in blocking, with three treatments and seven replications, totaling 21 plots. The treatments tested were whole corn plant silage (SM), BRS Capiaçu elephant grass silage (SC) and BRS Capiaçu elephant grass silage added 10% corn bran (SC+10%FM) based on natural matter. The experiment lasted 58 days (8 days of adaptation to the diets and 50 days of experimental period). The means were compared using the Tukey test at 5% probability. For the variables initial BW (PCi, P=0.8851), BW at slaughter (PCa, P=0.4261), hot carcass weight (PCQ, P=0.3456), hot carcass yield (WHR, P =0.4202), cold carcass weight (PCF, P=0.2923), cold carcass yield (RCF, P=0.2820), true yield (RV, P=0.7802), pH measured immediately after slaughter (pH0, P=0.0669), pH measured 24 hours after slaughter (pH24, P=0.1376), finishing (AC, P=0.9573), perirenal fat assessment (AGPR, P=0, 0791) and body condition score (BCS, P=0.4585) no statistical difference was observed between animals on different diets. Animals consuming corn silage had greater total weight gain (GPT, P=0.0018) and average daily gain (ADG, P=0.0018) than animals consuming capiaçu grass silages. There was no significant difference in the variables of fat thickness (EG, P=0.9134), rib eye area (AOL, P=0.0814) and carcass compactness index (ICC, P=0.2034) of the animals submitted to different diets. For the color parameters of the Longissimus dorsi muscle, a difference was observed in the a* coordinate (red intensity, P=0.0364), between animals submitted to corn silage and Capiaçu silage diets with 10% of FM, with average values of 15.39 and 13.69 for SM and SC+10%FM, respectively. Although some significant differences were observed, there were no losses in carcass and meat quality among the silages studied, with this we can conclude that silage from the BRS Capiaçu cultivar can be an alternative in feeding small ruminants to replace corn silage.
  • YOHANA ROSALY CORRÊA
  • Detoxified castor bean meal as an additive for sugarcane ensilage in sheep confinement.
  • Advisor : EDSON MAURO SANTOS
  • Date: Feb 26, 2024
  • Time: 08:00
  • Show Summary
  • The present study was developed through the conduction of an experiment, which generated information that will be presented in the form of two chapters. It is emphasized, initially, that the sugarcane used in the experiments to be described was ensiled with the addition of detoxified castor bean meal at four levels 0, 10, 20, and 40% (based on natural matter). In the first chapter, the experiment was conducted aiming to evaluate the intake, performance, and digestibility of male crossbred lambs fed diets containing ensiled sugarcane with increasing levels of detoxified castor bean meal. Twenty-four sheep, with an average body weight of 25 ± 1.5 kg and approximately four months old, were used, distributed in a completely randomized design with four treatments and six replications. The treatments consisted of the four levels of inclusion of castor bean meal in sugarcane silage. Dry matter intake (g/day, % BW, and g/BW^0.75), organic matter, crude protein, ether extract, total digestible nutrients, total carbohydrates, and non-fiber carbohydrates were recorded. A decreasing linear effect (P<0.05) of the levels of castor bean meal inclusion was observed for crude protein and ether extract. Dry matter intake, organic matter intake, neutral detergent fiber intake, total carbohydrate intake, and non-fiber carbohydrate intake were not influenced by the inclusion of castor bean meal in sugarcane silage. There was an effect (P<0.05) of the levels of castor bean meal inclusion on apparent digestibility of crude protein and ether extract. However, there was no effect (P>0.05) on the apparent digestibility of dry matter, organic matter, neutral detergent fiber, total carbohydrates, and non-fiber carbohydrates. Regarding performance, no effect of diets was observed on final body weight, total weight gain, average daily gain, feed 519 conversion ratio, and efficiency of feed utilization. Based on the absence of negative impacts on animal performance, it is recommended to incorporate up to 40% of detoxified castor bean meal in sugarcane silage diets for confined sheep for up to 60 days. In the second chapter, the objective was to evaluate the blood parameters and histopathological parameters of confined sheep fed sugarcane silage supplemented with levels of detoxified castor bean meal. The experimental design and conditions were the same as in the first chapter. Blood samples were collected on the first and 526 forty-eighth experimental days, and liver, kidney, and rumen samples were collected after slaughter for histomorphometric analyses. There was no effect (P>0.05) on muscle tissue thickness and rumen papillae height. However, epithelial thickness, keratinized portion, hemoglobin, hematocrit, and mean corpuscular volume showed significant effects (P<0.05) related to time and level of castor bean meal inclusion. No significant alterations (P>0.05) were identified in the blood count, leukogram, or in histopathological effects suggesting toxicity in animals due to the inclusion of castor bean meal in their diet. It is recommended to include up to 40% of detoxified castor bean meal for use in sugarcane silage diets for sheep for up to 60 days without causing 535 negative impacts on animal health.
  • MARIA ALYNE COUTINHO SANTOS
  • -
  • Advisor : JULIANA SILVA DE OLIVEIRA
  • Date: Feb 23, 2024
  • Time: 09:00
  • Show Summary
  • The regrowth age of tropical grasses intended for silage is a determining factor in the quality of the preserved material, considering that the nutritional characteristics change according to its height, as well as consumption, digestibility and animal performance depend on the quality of this material. Thus, the objective was to evaluate the effect of BRS Capiaçu grass silages harvested at different heights and added with corn bran to replace corn plant silage in the diet of sheep in confinement. Twenty-eight male animals were used, with no defined breed pattern, with an average initial live weight of 18kg, distributed in a randomized block design, with seven replications. The diets were composed of corn silage (CS), BRS capiaçu grass silage harvested at 4m height (CGS4m), capiaçu grass silage harvested at 4m + 10% corn (CGS4m+10%C) and capiaçu grass silage harvested at 2.5m height + 20% corn (CGS2.5m+20%C). A significant effect was observed for DMI, OMI, CPI, EEI, NDFI, NFCI, CC and TDN (P<0.0001), with CGS2.5m+20%C (43.04g/kg BW) and CGS4m (40 .44g/kg BW) showed the highest DMI values, while CS (29.53g/kg BW) showed the lowest value. For digestibility coefficients, statistical differences were also observed for all variables evaluated, where CGS4m+10%C and CS showed similarity in terms of NDFD, obtaining values of 590.95g/kg and 581.11g/kg, respectively. For TDN, the highest value was observed in CS (828.79g/kg), followed by CGS4m (750.04g/kg) and CGS4m+10%C (738.60g/kg), which did not differ from each other, while SGC2.5m+20%C presented the lowest value (687.04g/kg) for this variable. Animals consuming SGC2.5m+20%C (2.59L/day) and SGC4m (2.42L/day) demonstrated greater water intake via food, while animals fed CS showed greater water intake via drinking fountain compared to the others diets. For total water intake, no statistical difference was observed. For the performance variables, CS, SGC4m and SGC4m+10%M did not demonstrate a statistical difference for the TG, being 8.81kg, 9.90kg and 8.73kg, respectively. CS (4.25) and SGC4m+10%C (5.12) presented the best FC compared to the other diets. For FE, the diet containing CS (240.16) also stood out, while the others did not differ from each other. There was no effect for PF and ADG, which obtained averages of 35.39kg and 0.21kg/day, respectively. No statistical difference was observed for the time spent ruminating (419.58min/day), feeding (243.26min/day), idleness (777.08min/day) and chewing (662.90min/day). For feeding efficiency there was an effect in relation to g NDF/hour (P=0.0162), but no effect was observed for g DM/hour, as well as there was no effect for rumination efficiency. For the chewing variables, there was a statistical difference for chews/day (P=0.0003) and g DM/cake (P=0.0006). Therefore, it is recommended as an alternative to replacing corn silage, the use of BRS Capiaçu grass silage harvested 4m high and added with 10% corn.
  • PAULO DA CUNHA TÔRRES JÚNIOR
  • INFLUENCE OF TWO LEVELS OF FERTILIZATION ON THE PRODUCTIVITY OF THE SORGHUM OR CORN INTERCROSS WITH UROCHLOA BRIZANTHA CV MARANDU IN THE LIVESTOCK CROP INTEGRATION SYSTEM IN THE CERRADO MINEIRO
  • Advisor : EDSON MAURO SANTOS
  • Date: Feb 19, 2024
  • Time: 14:00
  • Show Summary
  • Crop-livestock integration (ILP) is an agricultural production system that combines animal husbandry with the production of agricultural crops, aiming to maximize natural resources, sustainability and economic profitability. The objective of this work was to evaluate the agronomic characteristics and the cost of producing corn and sorghum silage under two levels of fertilization in the crop-livestock integration system. The experiment was carried out at Fazenda Capim de Cheiro, in Paraopeba, MG, Brazil, in an Oxisol soil. Two agricultural crops were used (corn and silage sorghum) intercropped with Urochloa Brizantha cv. Marandu. With the respective consortiums: corn + grass with a high dose of fertilizer; sorghum + grass with a high dose of fertilizer; corn + grass with a low dose of fertilizer and sorghum + grass with a low dose of fertilizer. A randomized block design was used, with a 2x2 factorial design with two fertilizer levels and two crops, and five replications. The data were analyzed using the R software, using the F test. The highest production of natural matter was observed at the high dose level, with average values of 51,765.62 and 60,578.12 kg/ha, for the corn + grass and sorghum + grass, respectively. For the low dose of fertilizer, the highest production was observed for the sorghum + grass intercrop (31,993.75 kg/ha). The lowest costs per kilogram of dry matter were also observed at the highest level of fertilization, with values of R$ 0.30 and R$ 0.227, for the corn + grass and sorghum + grass consortia, respectively. At the end of the experiment, it was concluded that the high level of fertilizer dose guarantees a greater production of dry matter, regardless of the intercrop chosen, thus diluting the costs of recovering pastures.
  • LAIORAYNE ARAÚJO DE LIMA
  • Surface water sampling using modified Moore suabe: effect of using triplicates on the frequency and diversity of Salmonella enterica serovars.
  • Advisor : CELSO JOSE BRUNO DE OLIVEIRA
  • Date: Feb 19, 2024
  • Time: 08:00
  • Show Summary
  • The increasing number of studies reporting the occurrence of Salmonella enterica in non-recycled surface water warrants further investigations into their real role as potential sources of contamination. Considering the low concentration of Salmonella in water, the use of modified Moore’s swabs (MMS) has become a viable technique for the recovery of Salmonella enterica for this type of sample, enabling the sampling of larger volumes of water and reducing logistical costs. and problems. Here we report the unprecedented effects of using MMS replicates on the recovery and diversity of Salmonella enterica serovars. 270 water samples were collected in triplicate using MMS, totaling 810 swabs. MMS were cultured using standard microbiological procedures for isolation of S. enterica. Furthermore, we also tested the accuracy of enrichment broth-PCR as a screening method for S. enterica recovery. Furthermore, S. enterica serovars from a sample of 131 isolates were determined in silico after complete genome sequencing. The overall frequency of Salmonella enterica in water samples was 77.8% (210/270), while the frequencies observed in each individual replicate were 63.7, 62.6, and 61.1%. The use of triplicates resulted in higher (P<0.05) isolation frequencies of Salmonella enterica. Among the 210 samples containing S. enterica, 123 (58.6%) were positive in all triplicates. However, 50 (23.8%) samples were positive in two of three replicates, while 32 (17.6%) samples were positive in only one of three replicates. Enrichment-PCR broth cultivation showed high sensitivity (83.8%) and specificity (84.2%) in detecting viable Salmonella compared to conventional water culture, although no significant differences were observed between Tetrationate broth and Rappaport-Vassiliadis broth. Furthermore, the use of triplicates significantly increased the diversity of Salmonella serovars recovered from each sample. Our results indicated that the use of triplicates of 10L-MMS could significantly improve the recovery and diversity of S. enterica serovars in water samples. However, other aspects involving technical and cost limitations associated with the use of triplicates must be considered.
  • PALOMA EDUARDA LOPES DE SOUZA
  • USE OF MICROENCAPSULATED ACIDS WITH ESSENTIAL OILS IN THE DIETS OF LAYING HEN
  • Advisor : FERNANDO GUILHERME PERAZZO COSTA
  • Date: Jan 26, 2024
  • Time: 09:00
  • Show Summary
  • The use of antibiotics as growth promoters in animals is being restricted due to the development of bacterial resistance and posing risks to public health. Therefore, studies have intensified in search of alternative substances that cause similar or superior effects to antibiotics, which help in the development of the microbiota and intestinal morphology, obtaining better zootechnical results. The objective of this study was to evaluate the effect of supplementing microencapsulated acids with essential oils in the diet of laying hens. The experiment was conducted at the Ovo Novo farm, using a total of 600 laying hens of the Novogen Brown line from 30 weeks of age distributed in a completely randomized design with six treatments, 10 replications with 10 birds each. The treatments were: control diet, 200AD1: 200g/t of additive 1, 500AD1: 500g/t of additive 1, 200AD2: 200g/t of additive 2, 500AD2: 500g/t of additive 2 and AD3: 500g/t of additive 3. Bird performance was evaluated based on variation in body weight, viability (%), egg production (%), feed consumption (g/bird/day), egg mass (g/bird/day), the feed conversion per dozen eggs (kg/dozen) and the conversion per egg mass (kg/kg). For egg quality, egg weight (g), shell thickness (mm), yolk index, yolk color, yolk centralization, Haugh Unit, specific gravity (g/cm3), percentage of albumen (%), yolk (%) and shell (%), pH of the yolk and albumen. Regarding the development of the gastrointestinal tract, the pH of the organs (cecum, duodenum, jejunum, ileum, gizzard and proventriculus) and the weight of the organs and coelomic fat (gizzard, liver, small intestine, large intestine, fat, pancreas, proventriculus) were evaluated. and spleen). Data were analyzed using the 5% Tukey test and comparisons between means using orthogonal contrasts, using a significance level of P<0.05 for all analyses. The supplementation of microencapsulated acids with essential oils at a dosage of 500g/t of AD2 in the diets of laying hens proved to be more effective in maintaining zootechnical performance, egg quality, organ development and intestinal morphology.
  • PALOMA EDUARDA LOPES DE SOUZA
  • USE OF MICROENCAPSULATED ACIDS WITH ESSENTIAL OILS IN THE DIETS OF LAYING HEN
  • Advisor : FERNANDO GUILHERME PERAZZO COSTA
  • Date: Jan 26, 2024
  • Time: 09:00
  • Show Summary
  • -
2023
Description
  • BRUNA DOS SANTOS SOUZA
  • USE OF BENTONITE AS A CHEMICAL ADDITIVE IN CORN SILAGE
  • Advisor : EDSON MAURO SANTOS
  • Date: Nov 24, 2023
  • Time: 14:00
  • Show Summary
  • The objective was to evaluate bentonite inclusion doses and their effects on the fermentative profile, ensiling losses, microbial populations, aerobic stability and chemical composition of corn silage. The experiment was conducted in a completely randomized design, with treatments arranged in a 5×3 factorial scheme, with five doses of bentonite inclusion (0; 20; 40; 60 and 80 g/kg natural matter) in corn silage and three times storage period (30, 90 and 180 days), with four replications. There was an interaction between additive and storage period (P<0.0001) for BAL populations, filamentous fungi, yeasts, organic acids, pH, soluble carbohydrates, dry matter, ether extract and neutral detergent fiber contents and aerobic stability of the silages. There was an increasing linear effect (P<0.05) for BAL at 90 and 180 days of storage. There was an increasing linear effect (P<0.05) for filamentous fungi after 30 days of storage. There was a decreasing linear effect (P=0.0139) for yeasts after 30 days of storage, a quadratic effect (P=0.0010) in the 90-day period and an increasing linear effect (P=0.0002) after 180 days of storage. There was a quadratic effect (P=0.0010) for lactic acid at 90 days, a decreasing linear effect (P<0.05) for acetic acid at 90 and 180 days, and an increasing linear effect (P<0.05) for propionic acid at 90 and 180 days. There was a quadratic effect (P<0.05) for soluble carbohydrates at 30 and 90 days and an increasing linear effect (P=0.0017) at 180 days. There was an increasing linear effect (P<0.05) for DM during the storage period of 90 and 180 days. There was an increasing linear effect (P<0.05) for MM during the storage period of 30, 90 and 180 days. There was a decreasing linear effect (P=0.0024) for RMS during the 180-day storage period. There was an increasing linear effect (P=0.0001) for PE during the 90-day storage period. There was an increasing linear effect (P<0.05) for aerobic stability during the 90 and 180 day storage periods. There was a decreasing linear effect (P<0.05) for maximum temperature during the 90 and 180 day storage periods. It is recommended to use 20-60 g/kg of bentonite in corn silage for 180 days to improve quality, reducing losses and promoting aerobic stability, chemical composition and fermentation profile.
  • JOEDERSON LUIZ SANTOS DANTAS
  • BETTER THERMAL COMFORT AND ANIMAL WELFARE IN DAIRY GOAT PRODUCTION UNITS IN A SEMIARID EQUATORIAL REGION
  • Advisor : ROBERTO GERMANO COSTA
  • Date: Sep 27, 2023
  • Time: 08:00
  • Show Summary
  • Small-scale dairy goat farming in arid and semi-arid areas of the planet is one of the main sources of income for family farmers. However, environmental challenges in these ecosystems, such as thermal and nutritional stress, coupled with the low investment power of local breeders in disruptive technologies, are still the main causes of low productivity, in addition to poor levels of human and animal well-being. In this research, the condition of animal welfare in dairy goat production units in a semi-arid microregion of Brazil was characterized, in addition to evaluating the perception of breeders on topics related to animal welfare and inclination to implement mitigation strategies. of thermal stress. Finally, a thermal conditioning system with artificial shading + sprinkler for dairy goats in small-scale systems was proposed. In the first study, the “AWIN” animal welfare protocol was used with some adaptations for evaluations of 32 dairy goat farms located in a microregion of Cariri Paraibano (Latitude, -7º S). In the second study, a total of 24 lactating goats were distributed and maintained in three environmental conditions: 1) Shade projected by a shade screen, with a solar radiation blocking level of 50%; 2) shade (100% blocking) + water sprinkling; and 3) shade projected by a clay tile roof (100% solar radiation blocking). Meteorological variables and the goats' autonomic, behavioral, biochemical, hormonal responses and milk production were evaluated over 60 days. In the first study, of the total number of farms evaluated, 7 kept their goats in confinement, 22 in semi-confinement and 3 exclusively on pasture. Nutrition and management failures, including human-animal interaction, in addition to heat stress were the main risk factors for animal welfare found on farms. On all farms, at least one animal was detected with a change in one of these indicators. Lactating goats with very thin or fat body scores and overgrown hooves were more likely to experience heat stress, especially when they were evaluated in the afternoon. Most farmers interviewed have heard about animal welfare, perceive animals as sentient, and believe that their goats experience heat stress at certain times of the day. They also reported being willing to employ strategies to mitigate heat stress. In the second study, at times of greatest radiant thermal load, goats with shade + sprinkler availability had lower coat temperature (-4 ºC), skin temperature (-1 ºC), rectal temperature (-0.3 ºC) and frequency respiratory (-30 resp. min-1) compared to those maintained in treatments with only shade. Furthermore, lower levels of NEFA and cortisol were also observed in goats housed in the shady + sprinkled pen. These animals produced, on average, 400 g animal-1 day-1 more milk. In conclusion, thermal and nutritional stress are the main risk factors for animal welfare in goat milk production units in a semi-arid microregion of Paraíba. For the most part, breeders show that they are inclined to implement strategies to mitigate heat stress, despite reporting that they do not know how. Our results suggest the use of artificial shading + water spraying as the most effective way.
  • MARIA ISABELLY LEITE MAIA
  • Comparing the heat dissipation mechanisms in quails and laying hens
  • Advisor : EDILSON PAES SARAIVA
  • Date: Aug 4, 2023
  • Time: 09:00
  • Show Summary
  • In recent years, there has been a growing interest in animal welfare, driven by international market demands for products from production systems. In the case of laying hens, the thermal environment is crucial to their performance. Therefore, this study consists of two chapters. The first chapter aims to address: 1) The challenges of poultry farming in tropical climates; 2) Physiological responses of birds to thermal challenges; 3) Thermoregulation and thermal comfort in birds: mechanisms of body temperature regulation; 4) Heat exchange mechanisms in birds and their impact on thermoregulation; 5) Application of thermography as a tool for estimating animal thermal balance and heat flow. In the second chapter, the objective was to evaluate heat exchange through sensible and latent mechanisms, as well as to estimate the order of importance of different thermal windows in laying quails and hens. The study was conducted in the bioclimatic chambers of the Center for Agricultural Sciences at the Federal University of Paraíba (UFPB), Campus II. Two animal species, quails, and laying hens, were used at two temperatures (24°C and 5°C). Physiological and environmental parameters were collected three times a week for four weeks. The results of this study reveal significant differences in the use of sensible heat dissipation mechanisms between quails and commercial laying hens. Quails demonstrate greater efficiency in using sensible mechanisms, especially through convection, when compared to laying hens. Laying hens primarily use their feet as the main thermal window in warmer environments, while quails show greater effectiveness in using the lower wing area and feet to dissipate heat into the surroundings. These discrepancies indicate that these two bird species respond differently to the thermal environment in which they are housed.
  • CARLOS HENRIQUE DO NASCIMENTO
  • SUBSTITUTION OF BICALCIUM PHOSPHATE AND MEAT AND BONE MEAL BY BLEND OF ENZYMES AND ACIDS IN DIETS OF JAPANESE QUAILS (Coturnix Coturnix japonica)
  • Advisor : FERNANDO GUILHERME PERAZZO COSTA
  • Date: Aug 3, 2023
  • Time: 13:00
  • Show Summary
  • The study aimed to evaluate the effect of replacing conventional sources of calcium and phosphorus by an additive based on enzymes and organic acids in Japanese quail diets. A total of 144 32-week-old quails were distributed in a completely randomized design, in three treatments and six replications, with eight birds per experimental unit. The treatments were 1AD, FCO and FB. The experiment was divided into five cycles of 21 days. Three diets were formulated: 1AD, without the use of meat and bone meal and without dicalcium phosphate with 1% of the additive; FCO, without additive and without dicalcium phosphate with free use for meat and bone meal; and FB, without additive, without meat and bone meal with free use of dicalcium phosphate. Zootechnical performance, egg quality, bone quality, blood parameters and duodenum histomorphometry, jejunum histomorphometry and hepatic glycogen reserve were evaluated. The results were submitted to ANOVA and the means were compared by Tukey's test at 5% probability. The 1AD treatment showed greater results for UH, CG, RO, absorptive area of duodenum and jejenum, a higher concentration of liver glycogen and improved CAMO, CADZ. It can be concluded that the addition of a blend of enzymes and organic acids can be added to the Japanese quail diet in the proportion of 1kg/ton of quail feed without causing damage to the quality and performance of the birds.
  • WILLYANE DE SOUZA SANTOS
  • Performance and intestinal morphology of broiler chickens supplemented in ovo with threonine and glutamine.
  • Advisor : ALEXANDRE LEMOS DE BARROS MOREIRA FILHO
  • Date: Aug 3, 2023
  • Time: 08:30
  • Show Summary
  • The present research evaluated the effect of supplying the amino acids glutamine and threonine in ovo on the performance and intestinal morphology of broiler chickens. 480 eggs obtained from a commercial hatchery, from CobbMale breeders at 33 weeks of age, were distributed into four treatments, corresponding to four solutions supplemented in ovo (S1 = saline solution; S2 = 3.5% threonine solution; S3 = 3.5% glutamine solution and S4 = threonine + 3.5% glutamine solution. After hatching, the chicks were distributed in a completely randomized experimental design, with 4 replications of 10 animals each. The length and relative weight of the organs of the digestive system, productive performance and jejunum morphometry were evaluated. There was no significant difference for feed intake and body weight at 21 days of age. Birds supplemented in ovo with the combination of Gln + Thr showed greater weight gain and better feed conversion compared to the other treatments. Small intestine length, liver and gizzard weight were not different between treatments. Small intestine weight was greater for animals supplemented with the combination of Gln + Thr compared to animals inoculated with saline or threonine. The proventriculus weight was higher for glutamine treatment. There was no significant difference for villus height. Crypt depth differed significantly between treatments, with the lowest mean found in animals that received glutamine supplementation. Treatment with glutamine supplementation showed the highest mean for the variable villus:crypt ratio (V:C). The combination of glutamine + threonia and treatment with isolated threonine promoted an increase in the variable area of villi. Based on the available data, it can be concluded that in ovo supplementation of threonine associated with glutamine has the potential to improve performance, the development of organs of the digestive system and act trophically on intestinal development. However, additional studies are needed to validate these conclusions and deepen our understanding of the effects of these supplements.
  • GABRIELLE DOS SANTOS SILVA
  • HEMATOLOGY AND SERUM BIOCHEMISTRY OF DAIRY GOATS SUPPLEMENTED WITH MARMELEIRO EXTRACT (Croton blanchetianus)
  • Advisor : ARIOSVALDO NUNES DE MEDEIROS
  • Date: Jul 31, 2023
  • Time: 09:30
  • Show Summary
  • This research aims to evaluate the effect of food additives from Marmeleiro extract (Croton blanchetianus) on the blood parameters of dairy goats. The study was carried out at the Small Ruminant Research Unit of the São João do Cariri Experimental Station – CCA/UFPB 30 crossbred goats (Saanen × American Alpine), weighing on average 45 ± 5.0 kg and producing an average of 2 kg of milk /day, distributed in individual wooden pens, provided with individual drinkers and feeders with ad libitum access to water and experimental feed. The experiment lasted 79 days, 21 of which were for adaptation and 58 for data collection. The animals were distributed in a randomized block design, with three treatments based on the inclusion of C. blanchetianus extract. Obtaining the dry extracts was carried out through the process of exhaustive maceration, using ethanol. In treatment 1 without the addition of extract, in treatment 2 with the addition of 400 mg of C. blanchetianus extract, and in treatment 3 with the addition of 800 mg of extract. The animals were fed a standard diet between treatments, roughage: concentrate ratio, 45:55, composed of Tifton hay, ground corn, soybean meal, and mineral supplement, and received encapsulated plant extracts as a feed additive before feeding. in the morning. The blood parameters were evaluated: total proteins, albumin, globulin, urea, uric acid, creatinine, and blood urea nitrogen, in the protein profile; glucose, cholesterol, low and high-density lipoproteins and triglycerides in the energy profile; the enzymes aspartate aminotransferase, gamma-glutamyltransferase, alanine aminotransferase, and alkaline phosphatase, in the enzymatic profile; and total calcium, calcium, phosphorus, Ca:P ratio, magnesium, chlorine, sodium, potassium, Na: K ratio, and strong ion difference, in the electrolyte profile. Total proteins and globulin decreased significantly (P < 0.05) with the inclusion of the extract. A significant difference was observed for the phosphorus variable (P = 0.021), between the treatments that received C. blanchetianus extract at dosages of 400mg and 800mg, respectively, and for the sodium variable (P = 0.03) between the control treatment, which did not receive extract and the treatments that received extract. For the variables related to hematological parameters, no statistical difference was observed between the treatments (P < 0.05) with the inclusion of the extract. The extract of Marmeleiro (Croton blanchetianus) can be inserted into the diet of dairy goats as a phytochemical additive up to the dose of 800 mg without changes in blood parameters, where its concentrations did not cause hepatotoxic and nephrotoxic risks in the animals under study.
  • CLÁUDIO BALBINO DA CRUZ JUNIOR
  • EFFECT OF MARMELEIRO EXTRACT (Croton blanchetianus) ON INTAKE, DIGESTIBILITY AND PRODUCTION OF GOAT’S MILK
  • Advisor : ARIOSVALDO NUNES DE MEDEIROS
  • Date: Jul 28, 2023
  • Time: 14:00
  • Show Summary
  • The aim of this study was to evaluate the performance of dairy goats supplemented with the ethanolic extract of Marmeleiro (Croton blanchetianus), consumption, digestibility, production and milk composition. The study was carried out at the Small Ruminant Research Unit of the São João do Cariri Experimental Station – CCA/UFPB. Thirty crossbred goats (Saanen × Alpino Americano), weighing on average 45 ± 5.0 kg with DIM of 21.83 ± 6.663 and average milk production of 2 kg /day, were distributed in a randomized block design, in individual pens, equipped with individual drinkers and feeders. with ad libitum access to water and experimental diet. The animals were offered a standard diet, roughage: concentrate ratio, 55:45, based on Tifton hay, ground corn, soybean meal and mineral supplement, and received encapsulated extracts as a food additive. The extract was obtained from parts of the Marmeleiro tree (Croton blanchetianus). Standard diet; Standard diet + 400 mg; Standard diet + 800 mg. Obtaining the dry extract was carried out through the process of exhaustive maceration, using ethanol, which was supplied daily, one hour after the morning meal. The77 experiment lasted 79 days, 21 of which were for adaptation and 58 for data collection. Intake, apparent digestibility of dry matter and nutrients, and milk production were evaluated. The Marmeleiro extract did not affect the intake (P = 0.465) and apparent digestibility (P = 0.183) of dry matter and nutrients in the feed, there was also no difference and for milk production (P = 0.920) and feed efficiency (P = 0.651) of the animals. As for the physicochemical characteristics of the milk, the extract promoted a reduction for the total solids (TS) (P = 0.014) and non-fat solids (SNG) (P = 0.018) of the milk with the supply of 800mg of the extract. The supply of Marmeleiro extract (Croton blanchetianus) had no effect on the consumption, performance or on the chemical characteristics of the milk of goats
  • CÍCERO JORGE DE MEDEIROS
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  • Advisor : LEONARDO AUGUSTO FONSECA PASCOAL
  • Date: Jul 27, 2023
  • Time: 15:00
  • Show Summary
  • The objective was to evaluate enzyme supplementation in diets with nutritional reduction for piglets in the initial phase on performance, incidence of diarrhea, serum biochemical parameters, nutrient digestibility, organ weight, bone parameters, intestinal morphology and hepatic glycogen. Forty piglets, male and female, of the same commercial lineage and from a commercial farm, weaned at 26 days of age with an average initial weight of 7.85±1.27 kg were used. The animals were distributed in a randomized block design and (DBC) to control differences in initial weight in a factorial scheme (2X2), with four treatments and five replications. Treatments were arranged as follows: DCP – Positive control diet; DCPE -Diet positive control with enzymatic complex; DCN Nutritionally reduced diet; DCNE – Diet with nutritional reduction with enzymatic complex. There was no interaction effect (P>0.05) for the performance variables, however, there was an effect of diet and enzyme supplementation in periods II and III, the nutritional reduction increased the CDR and negatively affected AC, in both periods . Enzyme supplementation negatively affected weight gain and AC in period III. There was a lower incidence of diarrhea in the first period in animals fed the DCN diet when compared to the DCP. There was no influence of the diets on the serum biochemical parameters evaluated at 42 and 60 days of age. There was an effect of the diet with nutritional reduction (NCD) for phosphorus content in the blood at 42 days of age. There was a reduction in mineral matter and phosphorus digestibility coefficients when the animals received the diet with nutritional reduction, for neutral detergent fiber and acid detergent fiber coefficients were improved with enzymatic supplementation. There was an interaction of factors on the weight of the liver and pancreas, in such a way that the DCNE diet provided lower weight of the organs. There was no influence of experimental diets on the analyzed bone parameters. In the morphological parameters of the intestinal epithelium, it was observed that the nutritional reduction negatively affected the height of villi and absorptive area. It is concluded that the nutritional reduction of the diets negatively affected the piglets and that the enzyme supplementation (phytase and xylanase) was not enough for the nutritional challenge.
  • VALÉRIA MARINHO LEITE FALCÃO
  • INTESTINAL DIGESTIBILITY AND GENE EXPRESSION IN WEARNED PIGLETS SUPPLEMENTED WITH L-ARGININE AND DAIRY PRODUCT
  • Advisor : PATRICIA EMILIA NAVES GIVISIEZ
  • Date: Jul 27, 2023
  • Time: 08:30
  • Show Summary
  • The objective of this study was to evaluate the digestibility of nutrients in diets containing or not whole milk powder and L-arginine and intestinal gene expression for weaned piglets. Thirty-two piglets with an average weight of 5,159 kg ± 1,919 commercial strains (Embrapa MS115 X Topigs®) were used. The animals were distributed in a randomized block design to control initial differences in live weight, using a 2x2 factorial scheme, with two diets (7% whole milk powder and no whole milk powder) and two levels of supplementation on top of L -arginine in the diet (0% and 0.6%), in four treatments with four repetitions, each repetition being tried by two animals. The experimental diets were ordered in DS – Control diet (corn and soybean meal); DSA – Control diet supplemented with 0.6% L-Arginine; DL- Diet consisting of corn and soy bran containing dairy product; DLA- Diet consisting of corn and soybean meal containing dairy product, supplemented with 0.6% L-Arginine. The total apparent digestibility of nutrients and energy was evaluated from the digestibility test using partial collection with celite® as an indicator. The coefficient of digestibility of the nutrients and the digestible energy of the diet were higher when the animals were fed with diets containing dairy product, while those fed with a diet without dairy product added with arginine had lower values of dry matter digestibility. Regarding digestible nutrients, the addition of dairy product improved the digestibility of nutrients, and the addition of arginine in the plain diet produced digestible organic matter and digestible energy. Increased claudin and occludin gene expression in the jejunum of animals fed a dairy diet, and expression of the absorbed firm 2 protein gene in animals fed 0.6% arginine. Expression of the neutral amino acid transporter gene, CAT1, was not altered by diet or L-Arginine supplementation. Supplementation on top of diets containing whole milk powder showed no beneficial effect on digestibility or expression of gut barrier genes.
  • MARIA VICTÓRIA HENRIQUE GENUÍNO
  • Detection of selection signatures in Zebu cattle from Brazil and Asian continent.
  • Advisor : MARCOS ELI BUZANSKAS
  • Date: Jul 26, 2023
  • Time: 14:00
  • Show Summary
  • From technological advances in the area of genomics and bioinformatics, it became possible to study the genetic structure of breeds and understand the changes in the genome caused by the process of natural and artificial selection over the generations. Thus, the objective of this work was to identify selection signatures in Nelore, Gir, and Sindhi cattle from Brazil and Asian continent. A total of 173 Nelore animals from Brazil, 50 Nelore animals from India, 48 Gir animals from Brazil, 27 Gir animals from India, 49 Sindhi animals from Brazil and nine Sindhi Pakistan animals were used. All animals were genotyped with thehigh-density BovineHD BeadChip panel. For the detection of selection signatures, the XP-EHH, Rsb and Fst methods were used. The criterion of nonsliding windows, with a size of 50kb, was used to define the selection signature regions. Each window contained the average of the values of the methods used for the considered size of the window. The 40 largest means within windows for XP-EHH, Rsb, and Fst for each breed were considered for functional analyses. In this study, it was possible to observe selection signatures between populations of the same breed, but which differ in terms of geographic location. Both Brazil and Asian continent, differ in selection pressures and objectives. Thus, the identified selection signatures demonstrate how animal breeding programs are able to shape the genetic constitution of these breeds so that they are in conformity with the production systems. The identification of genes mainly related to adaptability, immunity, resistance to pathogens and parasites indicate that, even if these traits were already present in the first imported zebu cattle, certain alleles were fixed so that Nelore, Gir and Sindhi animals could adapt to the Brazilian production conditions.
  • PALOMA GABRIELA BATISTA GOMES
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  • Date: Jul 26, 2023
  • Time: 14:00
  • Show Summary
  • Using conventional concentrate feeds to overcome nutritional deficits can often be economically unfeasible in production systems, so an interesting alternative is to use available native forages with good nutritional value, such fava bean pods. In this context, the objective was to evaluate the inclusion of increasing levels of ground fava bean pods in place of ground corn in feedlot sheep diets. Twenty-eight sheep without racial pattern, with average initial live weight of 20.05 ± 0.44 kg, average age of 120 days, were distributed in an experimental design in randomized blocks (DBC), with seven replications. The diets consisted of Tifton-85 grass hay and concentrates (ground corn grain, soybean meal, wheat bran, crushed fava beans and mineral supplement), with treatments represented by the inclusion levels of crushed fava beans (0, 33, 66 and 100%) based on dry matter (DM). The supply of increasing levels of crushed fava bean pods in the diet of sheep linearly decreased the consumption of ether extract (P=0.0049). There was a decreasing linear effect on the apparent digestibility coefficients of dry matter (P=0.0354), organic matter (P=0.0384), crude protein (P=0.0016), ether extract (P=0.0361), neutral detergent fiber (P=0.0148) and total digestible nutrients (P=0.0039). Regarding the digestibility of non-fiber carbohydrates, there was an increasing linear effect (P=0.0003). No effect was observed for the performance variables (P>0.05). There was no statistical difference for the times spent feeding, ruminating and idling, ruminating and feeding efficiencies in relation to dry matter and neutral detergent fiber (P>0.05). For the variables of meric chewing, a quadratic effect was observed for the time of meric chewing per ruminated bolus (P=0.0218), daily number of meric chews (P=0.0240), number of meric chews per bolus (P=0 .0232) and number of meric chews per minute (P=0.0246). According to the results above, it is recommended to replace ground corn with ground fava beans in feedlot sheep diets up to 100%.
  • JOSÉ FABIO FERREIRA DE OLIVEIRA
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  • Advisor : ARIOSVALDO NUNES DE MEDEIROS
  • Date: Apr 14, 2023
  • Time: 09:00
  • Show Summary
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  • RAIANE DOS SANTOS SILVA
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  • Advisor : RICARDO ROMAO GUERRA
  • Date: Mar 9, 2023
  • Time: 14:00
  • Show Summary
  • The aim of this study was to verify whether phytase overdose in situations of thermal stress due to high temperatures can minimize the deleterious effects on egg production in Japanese quails. 720 Japanese quails were used in the production phase, housed in climatic chambers and distributed in a completely randomized design, in a 5x3 factorial scheme, with five phytase levels (0; 500; 1000; 1500; and 3000 FTU/Kg) and three temperature ranges (24; 30 and 36°C), totaling 15 treatments with six repetitions of eight birds each. The parameters evaluated for performance: feed intake (CR - g/bird/day), total egg production (PRO), egg weight (PO), egg mass (OM), feed conversion per egg mass (CAMO), and feed conversion per dozen eggs (CADZ). For internal and external quality: yolk height (AGEMA), albumen height (AALB), yolk weight (PGEMA), yolk percentage (PRGEMA), albumen weight (PALB), albumen percentage (PRALB), weight of bark (PCASCA), percentage of bark (PRCASCA), haugh unit (UH) and specific gravity (GE). Data were analyzed using R 4.2 software (2021), with regression being performed to compare phytase levels within each temperature range, and a Tukey test (p<0.05) to compare temperatures within each concentration of phytase. The parameters evaluated for organ weight: Spleen Weight (PB), Liver Weight (PF), Heart Weight (PC), and Coelomic Fat Weight (PGC). For performance we can see that there was no effect of treatments on the analyzed variables. However, there is a noticeable effect of temperatures on performance variables. Among the three temperature ranges analyzed, the 30°C range stands out over the others (24 and 36°C), this is due to the fact that animals submitted to a temperature of 30°C present CR similar to those of birds fed at the temperature referred to as comfort thermal (24°C), PRO superior to the other temperature ranges (24 and 36°C), as well as better CAMO and CADZ, even with egg weight similar to the other temperature ranges. Comparing temperatures between phytase levels, the 0FTU level (36°C) had the lowest value for AG. A quadratic effect was observed for PA (30°C), comparing the temperatures between the levels of phytase for PA, PG, PC, GE and UH, the environment of 36°C was the one that presented the lowest averages. For EC there was a linear effect (24 and 36°C), 0FTU showed the highest values of EC (24°C) and GEM (36°C) when comparing temperatures between phytase levels. For HU there was a linear and quadratic effect (36°C). There was a linear and quadratic effect for ALB (24 and 36°C) and a linear effect (36°C) for CASC. For PF there was a quadratic effect for temperatures 24 and 30°C. In PC, a linear effect was observed for phytase levels at 36°C. For PGC there was a linear and quadratic effect on the levels at the temperature of 30°C, this during the 2nd cycle. During the 4th Cycle PB showed a quadratic effect on temperature (36°), PF a quadratic effect on environments (24 and 30°C), PC linear effect on environment (36°), PGC linear and quadratic effect on environment of 36° W. It was concluded that quails can adapt to high temperature environments (36°C). However, when subjected to a temperature of 30°C, they can express better production rates and egg quality. Phytase, by making more nutrients available in the diet, at higher temperatures, an overdose (3000 FTU - temperature 30°C) provided the highest weights for PF and PGC.
  • DANIELA DA SILVA GOMES
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  • Advisor : PATRICIA EMILIA NAVES GIVISIEZ
  • Date: Mar 9, 2023
  • Time: 08:00
  • Show Summary
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  • KILMER OLIVEIRA SOARES
  • Effect of agrochemicals on the intestinal microbiota of bees.
  • Date: Feb 28, 2023
  • Time: 14:00
  • Show Summary
  • Although bees are responsible for ensuring the ecological stability of numerous environments, they are susceptible to various human activities that can result in the extinction of species due to environmental degradation, such as the use of agrochemicals. Africanized bees (Apis mellifera scutellata x spp.) play a significant role in the commercialization of beekeeping products in Brazil. However, very little is known about these bees, since they originated recently in Brazil (1956), through accidental crossing involving some European (Apis mellifera spp.) and African (Apis mellifera scutellata) bees. Considering the fundamental role of the intestinal microbiota of bees for health and, consequently, pollination of the various biomes, this thesis aims to investigate the composition of the intestinal microbiota of Africanized bees in relation to European and African bees, in addition to evaluating, experimentally, changes in the microbiota associated with exposure to agrochemicals. Chapter 1 (The honey bee gut microbiome: a review from the perspective of bees threatened by agrochemicals) deals with a review of the intestinal microbiome of bees and its changes associated with the use of agrochemicals. In Chapter 2 (Africanized honey bees (Apis mellifera scutellata x spp.) gut microbiota: is it similar to African or European bees?), we present a comparative evaluation in silico, using 16S rRNA gene sequencing data from 126 bee intestinal samples European (n=34), African (n=82) and Africanized (n=10). The results demonstrated that the intestinal microbiota of Africanized bees is more similar to that of European bees, although the relative abundance of the genus Snodgrassella was higher in Africanized and African bees compared to European bees. The genus Gilliamella was more abundant in Africanized bees compared to African bees. Chapter 3 (Tetracycline Exposure Alters Key Gut Microbiota in Africanized Honey Bees (Apis mellifera scutellata x spp.) aimed to experimentally evaluate the effect of exposure to the antibiotic tetracycline in Africanized bees. (CON: fed daily with syrup (10 g) composed of a 1:1 solution of demerara sugar and water, plus a solid protein diet (10 g) composed of 60% soy extract and 40% sugar syrup) and exposed group (TET: fed identically to CON, but with the addition of tetracycline hydrochloride at a dose of 450 μg/g to sugar syrup) were obtained from each group before (day 0) and after exposure to tetracycline (days 3, 6 and 9) After sequencing the V3-V4 region of the 16S rRNA gene in Illumina MiSeq, and processing through QIIME2 and DADA2, the results showed significant differences in the composition (Jaccard) and diversity (Shannon) of the microbiota intestinal, sen such differences become more accentuated as the exposure time increases. TET reduced the relative abundance of Bombella and Fructobacillus, along with a decrease in essential microbiota such as Snodgrassella, Gilliamella, Rhizobiaceae and Apibacter. As these microorganisms are critical for nutrient metabolism and pathogen defense, it is possible that their decrease could negatively affect bee health. Chapter 3 (The effects of zinc and laboratory emerge date on honey bee gut microbiota) refers to an experimental study on the effect of zinc on the microbiota of European bees. Zinc is often found in agricultural runoff as it is used in fertilizers, insecticides and food additives. Studies on the supposed effects of zinc on bee health are, however, scarce. In this study, bees were exposed to seven concentrations of zinc (0, 50, 100, 250, 500 or 1000 mg/L). Survival was only minimally impacted (>89% survival) at zinc concentrations up to 100 mg/L. To evaluate the effects of zinc on the intestinal microbiota, the bees were exposed to concentrations of 5 or 100 mg/L of zinc. Gut samples were collected before (day 0) and after exposure (days 3, 6 and 9). Abdominal contents were subjected to DNA extraction and sequencing of the V3-V4 region of the 16S rRNA gene (V3-V4) on an Illumina MiSeq. Sequences were filtered and processed through QIIME2 and DADA2. Although exposure to zinc has minimal effects on bacterial DNA concentrations and absolute microorganism counts, a significant difference in concentrations was observed between the different days of emergence (moment that the bee emerges from the brood comb). In this situation, a decrease in bacterial concentrations was observed. Significant differences in taxonomic diversity and abundance were observed in bees exposed to the highest concentration of zinc (100 mg/L), in which a reduction of several beneficial taxa (Lactobacillus, Rhizobiaceae, Gilliamella) and an increase of Paenibacillus, a taxon potentially pathogenic. The results indicate that exposure to zinc, even at relatively low levels, can negatively affect bee health, even if survival is not drastically affected.
  • LUCAS NUNES DE MELO
  • EVALUATION OF THE ADDITION OF ACIDIFYING AND PHYTOGENIC ADDITIVES IN THE FEED OF BROILER CHALLENGED WITH EIMERIA
  • Advisor : FERNANDO GUILHERME PERAZZO COSTA
  • Date: Feb 28, 2023
  • Time: 14:00
  • Show Summary
  • Organic acids and essential oils have been used in the poultry industry, and have proven to be an effective alternative as growth promoters, protecting birds against enteric diseases and improving performance. However, the results found are still very inconsistent and the present study aims to evaluate the effect of adding organic acid alone or in association with a phytogenic additive in the diet of broiler chickens challenged with Eimeria sp. 1280 male Cobb 500 chicks aged 1 to 40 days were used, distributed in a completely randomized design with 8 treatments and 8 replications of 20 birds each. Treatments consisted of a negative control diet and a 2 x 3 factorial scheme, with and without phytogenic additive supplementation and three levels of microencapsulated sodium butyrate. Essential oil and sodium butyrate supplementation influenced cloacal pouch weight (P<0.05), reduced the number of oocysts excreted into the environment on the 10th day of age (P<0.05) and influenced villus height, depth of crypt and V:C ratio in the duodenum of birds. It did not influence the productive performance of the birds, the relative weight of the spleen and thymus and the intestinal morphometry variables in the jejunum and ileum (P>0.05). The addition of microencapsulated sodium butyrate in association with a mixture of oregano, thyme and cinnamon essential oils is able to improve immune function and prevent diseases such as necrotic enteritis and coccidiosis, in addition to preserving the integrity of intestinal morphometry and the productive performance of chickens cuttings challenged with eimeria sp.
  • TAMIRES MARCELINO DA SILVA FELIX
  • EVALUATION OF SYNTHETIC METHIONINE LEVELS AND SOURCES ON THE PERFORMANCE AND EXPRESSION OF METABOLISM GENES AND JEJUNAL TRANSPORTERS IN CHICKENS
  • Date: Feb 28, 2023
  • Time: 09:00
  • Show Summary
  • Methionine is an essential amino acid for poultry, being the first limiting amino acid in commercial broiler diets, along with cysteine. The metabolic processes of sulfur amino acids generate fundamental metabolites for several physiological processes, such as the synthesis of body tissues, antioxidative functions, cell division, taurine production, activation of mTORC1 and regulation of the expression of genes involved in the metabolism of birds. For methionine supplementation in deficient diets, DL-Methionine (DL-Met) or DL-Methionine Hydroxy Analogue (DL-HMTBA) is usually used, these have different chemical and physical structures, interfering with absorption and subsequent metabolism. Therefore, a systematic review was initially carried out in order to present an overview of the studies that evaluate the influence of supplementation with sulfur amino acids using DL-Met, DL-HMTBA or L-Met, on gene expression in broiler chickens. For this, a complete search was performed in electronic databases, using a combination of words by the PICo method (Population, Interest and Context) with terms related to broiler chickens, methionine and gene expression. The search was carried out in the Scopus, Web of Science and PubMed databases through the collection of the CAPES Periodicals Portal. The resulting articles were exported to a reference management program and subjected to various selection criteria, which ranged from a total of 596 to 15 articles. From the review it was possible to obtain an overview of the studies that evaluated gene expression in broiler chickens. With this, an experiment was carried out with the objective of evaluating the effects of DL-Met and DL-HMTBA in three levels of supplementation in the performance, expression of genes of the metabolism of sulphurous amino acids and expression of methionine transporters. For this, 450 chicks were distributed in a completely randomized design in a 2x3 factorial scheme, with two sources of methionine (DL-Met and DL-HMTBA) and three levels of supplementation (deficiency, requirement and excess), totaling 6 treatments with 5 replications. of 15 birds each. The experiment was carried out at the Poultry Laboratory of the Center for Human, Social and Agrarian Sciences, Campus de Bananeiras/UFPB. The evaluated parameters were: weight gain (g), feed intake (g), feed conversion (g/g), mRNA expression of genes (MAT1, MTR, BHMT, MTRR, CBG and GSS), mRNA expression of glucose 8 transporters (SGLT1, GLUT2 and GLUT5), and mRNA expression of methionine transporters (SAT1, SAT2, SAT3, ATB0,+, y+LAT1, y+LAT2, LAT1, B0AT, b0,+AT and rBAT) in the jejunum of broilers. DL-HMTBA increased the expression of BHMT (p=0.0072) and MTRR (p=0.0003) in the jejunum of birds. Methionine deficiency increased the expression of BHMT (p=0.0805) and MTRR (p=0.0018). Higher GSS expression was observed in birds that were supplemented with DL-HMTBA (p=0.0672). Birds fed with DL-HMTBA showed worse weight gain (p=0.0117) and higher feed conversion (p=0.0170); methionine deficiency resulted in higher feed intake (p=0.0214), lower weight gain (p<0.0001) and consequently higher feed conversion (p<0.0001). The use of DL-HMTBA increased the expression of SGLT1 (p=0.0065), SAT1 (p=0.0167) and y+LAT2 (p<0.0001). Methionine deficiency resulted in increased expression of SGLT1 (p=0.0004), GLUT5 (p=0.0001), SAT2 (p=0.0064), y+LAT2 (p=0.0677) and rBAT (p =0.0800). The use of DL-Met increased LAT1 expression (p=0.0420). It is preferable to carry out sulfur amino acid supplementation with DL-Met at the requirement and DL-HMTBA above the requirement.
  • ADIEL VIEIRA DE LIMA
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  • Advisor : FERNANDO GUILHERME PERAZZO COSTA
  • Date: Feb 28, 2023
  • Time: 08:00
  • Show Summary
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  • JORGE LUIZ SANTOS DE ALMEIDA
  • USE OF VITAMIN D FOR SWINE AND PERSPECTIVES OF USE IN DIFFERENT PRODUCTION PHASES
  • Date: Feb 27, 2023
  • Time: 14:00
  • Show Summary
  • This thesis work was carried out to investigate vitamin D supplementation for sows. In this sense, a literature review on the subject was carried out, a systematic review with the survey of articles that evaluated vitamin D supplementation in pigs and an experiment with the use of vitamin D for sows. Vitamin D is part of the class of fat-soluble vitamins and can be supplied via the diet usually in its inactive forms, including vitamin D2, D3, or 25(OH)D3, forms that are activated by two hydroxylation steps that occur in the liver and blood vessels. kidneys. Vitamin D3 plays a role in several crucial processes in animal metabolism. It influences the composition of the intestinal microbiota, being able to mitigate intestinal damage and protect against the induced inflammatory state of challenged piglets; it can alter the proliferation of muscle-derived myoblasts, improving skeletal muscle development in the swine fetus; and is capable of inducing gene expression of the antimicrobial peptide cathelicidin, capable of fighting bacterial and viral infections. Therefore, it is understood that vitamin D concentrations greater than 45μg/kg can meet a need that goes beyond the common routes of vitamin D. The search was carried out in the Scopus, Web of Science and PubMed databases through the CAPES Portal de Periódicos collection. The resulting articles were exported to a reference management program and submitted to several selection criteria, which started from a total of 1997 articles and were filtered until reaching 57 articles. Studies were classified by year of publication, with 67.1% of searches found between 2010 and 2020. The United States represented the country with the highest number of publications (31 articles). The most used source of vitamin D was D3, followed by 25(OH)D3 with 38.6 and 17.1% respectively. The most studied rearing phase was piglets in the nursery, where 25(OH)D3 was the most present source of vitamin D. Most of the studies occur mainly in critical periods, when the demand for vitamin D may be higher; the intensification of the use of 25(OH)D3 as a more efficient source in these periods demonstrates the relevance of vitamin D in swine nutrition. The systematic review can present an overview of studies, exploring which phases were most investigated and which sources were most explored in the articles, leaving the possibility of new scientific research, serving as a starting point for studies that want to observe results that have not yet been explored. The objective of the experiment (chapter 3) was to evaluate the supplementation of high levels of 25(OH)D3 in matrices and reflex in the progeny. For this, a total of 250 primiparous and multiparous sows with an average body weight of 204.78 kg were used, arranged in five treatments with ten repetitions. All experimental diets contained a baseline amount of vitamin D3 (45μg/kg of D3) and the experimental treatments consisted of adding 25(OH)D3 at five increasing levels (0μg/kg 25(OH)D3; 25μg/kg 25(OH)D3; 50μg/kg 25(OH)D3; 75μg/kg 25(OH)D3 and 100μg/kg 25(OH)D3. The productive performance of the sows were evaluated, as well as the reflection of the maternal diet on the productive performance of the litters, the serum levels of vitamin D metabolites in the sows and piglets, serum immunoglobulins A, G and M of the sows and the fatty acid profile of the milk and colostrum. 25(OH)D3 supplementation improved the sow's vitamin D status after parturition and immunoglobulin levels at parturition and weaning. The performance of the sows was not affected, however, the development of the piglets was influenced by vitamin D supplementation in the mother's diet. The profile of fatty acids in milk can be indicative of an effect on the outcome in piglets and should be further investigated.
  • PAVLOS VINICIUS DO NASCIMENTO
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  • Advisor : EDILSON PAES SARAIVA
  • Date: Feb 27, 2023
  • Time: 13:00
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  • In general, the thermal environment is considered the main factor that affects animal production, as its effects are directly reflected in the productive parameters of these animals. However, in dogs, mostly bred for companionship, the available information is not as clear as to the influence of the thermal environment on the well-being of these animals. Dogs are homeothermic animals, therefore, there must be a balance between the thermal energy originated and the thermal energy lost, so that they can maintain a relatively constant internal temperature. However, they are very sensitive to changes in temperature and it is extremely difficult to define a thermoneutral zone for this animal species due to the number of existing breeds. However, dogs represent the largest pet population in Brazil, and this is largely due to the ability developed by them to cooperate and help humans, that said, it was proposed to address the factors that affect the thermoregulation of domestic dogs raised in Brazil and evaluate Thermoregulatory responses of domestic mongrel dogs (NDB) during and after walking outdoors with exposure to different levels of solar radiation. The thermoregulation of 5 dogs (3 males and 2 females) castrated and healthy was evaluated during and after walking outdoors during the 3 periods of the day: morning, afternoon and evening. 135 collections were performed, lasting 42 minutes each. Physiological, behavioral and meteorological variables were collected and all statistical analyzes were performed using R 4.1.0. The results show that with the increase of BGTSun, the regulation of the body temperature becomes more and more difficult for the animal, that no matter how much it has maintained its body temperatures at narrow levels of variation, the costs were reflected in the increase in the RF and in the increase in TSkin. From the development of a thermal comfort index, it was possible to estimate a critical value for RF: 287 breaths per minute, since, from that moment on, there is an increase in the depth of breathing, thus increasing the tidal respiratory volume, which consequently leads to the animal to hyperventilate, causing a disturbance in the gas exchange process. Thus, it is valid to assume that mongrel dogs (NDB) when exposed to high levels of thermal load with direct sun exposure have an expensive expense to keep their body temperatures within narrow levels of variation.
  • EUDES FERNANDO ALVES DA SILVA
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  • Advisor : PATRICIA EMILIA NAVES GIVISIEZ
  • Date: Feb 24, 2023
  • Time: 08:00
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  • The objective was to evaluate the effects of inclusion levels of cupuassu seed meal on intestinal morphology, goblet cell count and mRNA gene expression of the MUC2 gene and intestinal nutrient transporters in free-range chickens. For the experiment, 300 chicks from the Label Rouge lineage were randomly distributed in five treatments corresponding to the levels of inclusion of cupuaçu seed meal in the diet (0, 7, 14, 21 and 28%), with five replications of 12 animals each . At 14, 28 and 42 days, 5 birds per treatment were randomly chosen and sacrificed. From these birds, samples were collected from the 2 intestinal segments (jejunum and ileum) for histological analyses, at the same time that samples from the jejunum were also collected for analysis of the MUC2, SGLT1, GLUT2, GLUT5, PepT1, ASCT1, B0at and FABP1 genes using qPCR, by real-time polymerase chain reaction (qPCR). Data were submitted to linear and quadratic regression analysis, means were compared by Dunnet's test at 5% significance level. Gene expression data were subjected to analysis of variance and means compared by Tukey and Dunnet test at 5% significance level. All analyzes were performed using the statistical program R (2008). From the results of the regression analysis, a decreasing linear effect was observed for the villus height in the jejunum at 14, 28 and 42 days; increasing linear effect for crypt depth in jejunum and ileum at 14 and 42 days and for muscle layer thickness and goblet cell count in jejunum and ileum at 28 and 42 days. By the Dunnet test, no significant differences were observed for any of the variables evaluated between the animals in the 0% and 7% bran inclusion groups at 28 days in the ileum. Similar to this, no significant differences were observed in the relative expression of the MUC2 gene at 14 days of age. Our results indicate “downregulation” in the relative expression of all intestinal transporters at 7 days of life (P<0.05), and this behavior was also observed at 28 days of life for transporters SGLT1, Pept1 and B0at (P<0 ,05) The relative expression of GLUT5 and FABP1 mRNA showed upregulation at 28 and 42 days. Despite this, the animals in the control group and in the group fed with 7% cupuassu meal inclusion showed similar expression patterns for SGLT1, GLUT2, GLUT5, PepT1, B0at and FABP1 (at 14 days); GLUTs 2 and 5, Pept1, B0at and ASCT1 (at 28 days) and GLUTs 2 and 5 and SGLT1 (at 42 days). The negative effects caused by the high levels of inclusion of this bran can be attributed to the negative effects that high concentrations of fiber have on the digestive physiology of the birds, which can cause an increase in the viscosity of the digesta and damage to the intestinal epithelium, leading to an increase in the extrusion process. or cellular desquamation at the apex of the villi and villous atrophy. As a way of replacing the extruded cells, there is an increase in the rate of cell proliferation in the crypts and cell migration along the crypt-villus axis. The “downregulation” effect may be related to the negative effects that high levels of fiber have on birds, especially young ones, in addition, birds do not have enzymes to digest fiber. As cupuaçu is rich in fructose, the increase in GLUT5 expression may be a response to the increase in fructose concentration in the diet. Despite this, moderate levels of this bran can bring benefits to the birds, this may be related to a possible modulation capacity of the microbiome and by favoring the fermentative processes that can generate products that can be used by the bird. In view of this, moderate levels of cupuaçu seed meal (7 and 14%) can be an alternative for feeding free-range chickens, since they do not alter the intestinal variables evaluated when compared to the group without cupuaçu meal inclusion.
  • ALMY DE SÁ CARVALHO FILHO
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  • Advisor : CELSO JOSE BRUNO DE OLIVEIRA
  • Date: Feb 15, 2023
  • Time: 08:00
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2022
Description
  • ELIAS LEOCADIO DOS SANTOS NETO
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  • Advisor : EDILSON PAES SARAIVA
  • Date: Nov 28, 2022
  • Time: 15:00
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  • The aim of this study was to evaluate the effect of black soldier fly (Hermetia illucenss) larvae meal on dog’s oral health through culture of volatile sulfur compounds (VSC) producing bacteria culture; breath malodour scoring and oral microbiota. Two isonutritive extruded dry foods were produced, one containing the defatted meal of black soldier fly larvae (FI) as the main source of protein and the other containing low-ash poultry byproduct meal (FV). Eight beagle dogs, males and females, mean age 3.2 ± 1.4 years, mean weight 10.88 ± 1.4 years were used in a cross-over design, with two periods of 50 days with half of the animals. consuming FI diet and the other half consuming FV diet. At the end of the first period, the animals underwent washout period (7 days) and the treatments were inverted in the subsequent period. The animals were fed twice daily (09:00 am and 04:00 pm) according to the maintenance energy requirement proposed by the NRC (2006). Water was provided ad libtum. Dental plaque was collected from the dogs before and after 50 days of diet consumption, for VSC producing bacteria culture by the plate counting method. The breath score was assessed by an olfactory sensory scale and the oral microbiota was analyzed in the dog’s saliva by sequencing the 16s region of the rRNA gene at the end of each period. There was a significant reduction in VSC producing bacteria CFU (P=0.047) between the beginning (day 0) and after 50 days of consumption for the FI treatment. However, for the characterization of the breath score, there was only a tendency towards difference between treatments (P = 0.097), with mean 2 for the FV treatment (minimum 1 and maximum 4) and mean 1 for the FI treatment (minimum 0 and maximum 2). There was a significant difference (P<0.0378) between treatments in the relative abundance of Moraxella sp.002224245 species when they received the FI treatment. This bacterial group is identified as an indicator of oral health. The consuption of black fly larvae meal decreases VSC producing bacteria CFU and positively modulates the oral microbiota, which can contribute to the reduction of halitosis and the maintenance of oral health.
  • HACTUS SOUTO CAVALCANTI
  • SUGARCANE SILAGES ADDED WITH COTTONSEED CAKE AND MICROBIAL INOCULANT FOR FEEDLOT OF LAMBS
  • Date: Sep 26, 2022
  • Time: 08:00
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  • The objective was to evaluate the fermentative and nutritional profile of sugarcane silages added with cottonseed cake (20% fresh matter), heterofermentative inoculant (Weissella cibaria) and a combination of both, which were used in the diet of feedlot lambs to evaluate performance, intake, digestibility and ruminal parameters. Two experiments were carried out. The first experiment was carried out in a completely randomized design in a 4 x 2 factorial scheme (four additives and two opening times), with 4 replications, totaling 32 experimental units. In the second experiment, a completely randomized design was used with two diets consisting of (1) sugarcane silage (control) and (2) sugarcane silage with 20% cottonseed cake. A total of 26 lambs were used, 13 for each treatment, which were confined for 60 days at a private farm in São José dos Cordeiros - Paraiba. An interaction between additive x opening time was observed for pH, where the silages added with cottonseed cake showed higher values (3.6 to 3.8) in relation to the others (<3.6). There were isolated effects for additive and opening time for organic acids, with higher production of acetic acid from silages in the last opening time (240d). Dry matter recovery was higher in silages with cottonseed cake (≈92%), followed by silage inoculated with Weissella cibaria (85%) and control (63%). The silages added with cottonseed cake showed higher values of dry matter (≈38%), crude protein (≈12%), ether extract (≈5%) and lower fiber content, if compared to the others. The inclusion of cottonseed cake in silages is recommended for fermentation improvements and greater dry matter recovery, with a plus of protein supply, however, the exclusive application of the inoculant is a viable possibility because it increases dry matter recovery compared to control silage. In the second experiment, it was observed that dry matter intake did not differ between treatments (≈1620 g/kg), however, dry matter digestibility (64.75%) and average daily gain (202.88 g/day) was higher in diets containing sugarcane silage added with cottonseed cake (P<0.05), resulting in greater total weight gain (8.11 kg) (P<0.05). The animals fed diets containing sugarcane silage added with cottonseed cake showed a lower acetate: propionate ratio (4.2 vs. 2.0 mmol/L) and higher blood glucose content (44 vs. 35 mg/dL). Diets containing sugarcane added with cottonseed cake promoted fermentative and nutritional improvements, with better preservation of nutritive value, resulting in better productive performance of confined sheep.
  • ANDERSON SAMUEL SILVA
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  • Advisor : EDSON MAURO SANTOS
  • Date: Sep 16, 2022
  • Time: 14:00
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  • EDWILKA OLIVEIRA CAVALCANTE
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  • Advisor : SEVERINO GONZAGA NETO
  • Date: Aug 31, 2022
  • Time: 08:00
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  • The objective of this study was to evaluate the effect of chemical and biological treatments on the value of cotton gin trash. The study was divided into three phases, namely: Test I - comprised the doses of NaOH and urea on different days of treatment with the result evaluated through chemical composition and in vitro dry matter digestibility (IVDMD); Test II – the best results of test I were submitted to doses of exogenous fibrolytic enzymes (EFE) and evaluated by chemical composition and IVDMD; Test III – the best results obtained in test II were submitted to carbohydrate fractionation, in vitro gas production and in situ degradability. The design adopted was completely randomized, with four replications. For Test I, there was a reduction in the concentration of NDF (P <.0001) and ADF (P <.0001), and increase in the concentration of CP (P <.0001) and IVDMD with higher doses (4.0 and 6.0%) of urea. The treatment time of 28 days allowed a reduction in the concentration of NDF (P <.0001) and ADF (P <.0001), and increase in IVDMD concentration (P=0.01). There was a reduction in the concentration of NDF (P <.0001) and ADF (P <.0001), and an increase in IVDMD (P <.0001) with higher doses (3.0 and 4.5%) of sodium hydroxide. In test II, there was interaction treatment with urea × enzyme dose for NDF concentration (P <.0001), ADF (P = 0.02) and for IVDMD (P = 0.03). There was interaction of the treatment protocols with sodium hydroxide × enzyme doses for IVDMD (P = 0.001). In test III, the urea-based protocols reduced (P <.0001) the concentration of total carbohydrates. The NaOH-based protocols increased (P <.0001) the participation of non-fibrous carbohydrates. All protocols increased (P=0.0005) the cumulative production of gases in the fast phase (Vf1), but kept stable (P=0.054) the degradation rate Kd1 and Kd2 (P=0.66). Shorter colonization time (P<.0001) was observed in NaOH-based protocols. The cotton gin trash showed an improvement in its nutritional value when submitted to chemical and biological treatments.
  • HEMILLY MARQUES DA SILVA
  • INTESTINAL MORPHOPHYSIOLOGY OF EUROPEAN QUAILS.
  • Advisor : PATRICIA EMILIA NAVES GIVISIEZ
  • Date: Aug 30, 2022
  • Time: 08:30
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  • Information on the intestinal morphophysiology of quails is still scarce, despite the increasing importance of quail production. This study was carried out to assess the intestinal morphology and the gene expression of carbohydrate and protein transporters in European quails (Coturnix coturnix): sodium-glucose co-transporter 1 (SGLT1), fructose transporter (GLUT5), glucose transporter (GLUT2), neutral amino acid transporter (ASCT1), sodiumdependent neutral aminoacid transporter (B0AT1) and peptide transporters (PEPT1). European quails were sampled at 7, 14 and 21 days of age (n=75 per age) for histology and transporter expression suing real time polymerase chain reaction (qPCR). Morphology and gene expression data were submitted to variance analysis and means were compared by Tukey’s test at 5%. Villus height and area were higher in the duodenum, although between 7 and 21 days there was a linear increase in all segments. Crypt depth increased in duodenum and jejunum from 7 to 14 days and stabilized until 21 days; whereas it increased until 21 days in the ileum. Interestingly, although no evident stress or intestinal disease, goblet cell counts were higher in jejunum and ileum when compared to duodenum. SGLT1, GLUT5, GLUT2 and PEPT1 expression was different between ages. It was concluded that the intestinal tract showed significant morphological and physiological changes during the rearing phase (1-21 d) and these are dependent on sampling age.
  • MAYLANE RAYANE BRITO DOS SANTOS
  • EXPRESSION OF MUCIN AND INTESTINAL TRANSPORTERS AND MICROBIAL PROFILE OF CHICKS OF DIFFERENT LINEAGES CHALLENGED WITH SALMONELLA ENTERITIDIS
  • Date: Aug 29, 2022
  • Time: 08:30
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  • The objective was to evaluate and compare the composition and diversity and expression of mucin and nutrient transporters in two chicken strains. Microbiome composition was investigated in birds of two strains by sequencing the 16S ribosomal RNA (rRNA) gene at 24, 96 and 168 hours after inoculation (hpi) with S. Enteritidis or sterile culture medium in two strains of chickens. Jejunal mucosa samples were collected for RNA extraction and later evaluation of the mucin gene (Muc2), the intestinal glucose transporters (Sglt1, Glut2 and Glut5) and the amino acid transporter (Asct1) and di-tripeptide transporter (PepT1) . According to the Shannon index, referring to the time after inoculation, it shows that the alpha diversity index is significantly lower in the treatments with 24h (P<0.05). The phyla Proteobacteria and Firmicutes were more dominant at 24, 96 and 168 hpi, there is greater abundance of Proteobacteria in relation to Firmicutes, the opposite occurs at 96 and 168 hpi. There was no significant effect at 24 hpi for intestinal transporters, however expression was upregulated for Muc2 in Label. In addition, there was lineage challenge interaction in the period of 168 hpi for Glut2. The strains apparently have different mechanisms in intestinal transport when challenged with S. Enteritidis.
  • PATRICIA RODRIGUES DE LIMA
  • Nutritional and bioeconomic responses of Santa Inês crossbred ewes in feedlot subjected to reduced water supply
  • Date: Aug 26, 2022
  • Time: 14:00
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  • Water scarcity is an alarming problem affecting humans and livestock worldwide, especially in arid and semi-arid regions. The native and naturalized sheep of these regions have developed adaptive mechanisms that allow them to produce in situations of water scarcity. The objective of this study was to evaluate the effect of reduced water supply on nutrient intake and digestibility, ingestive behavior, water and nitrogen balance, and bioeconomic performance (performance and economic indicators) of sheep under confinement. Thirty-two Santa Inês crossbred ewes with a live weight of 32.2 ± 7.4 kg and age of 2.3 ± 0.99 years were used. They were distributed in an entirely randomized design with four treatments (ad libitum water (Control - 100% intake), 80; 60 and 40% supply from the control group) and eight repetitions per treatment. The experiment lasted 72 days (14 for adaptation and 58 for data collection). Variables were subjected to normality test and regression analysis using PROC REG of the Statistical Analysis System, effects were considered significant when P>0.05. Nutrient intake and digestibility coefficients, mineral intake, ingestive behavior and performance were not influenced by water offers (P>0.05). A linear decreasing effect was observed for free water intake, total water intake (P<0.001), total urine output (P=0.008), water excreted via urine (P=0.006) water excreted via faeces (P=0.006), total water loss (P<0.001), water absorbed (P<0.001), for the ratios FWI/DMI, FWI/CPI and FWI/NDFI (P<0.001) and nitrogen excreted via faeces (P=0.002). There was an increase of 5.84% and 7.46% in feed costs at 60% and 40% water supply, respectively. There was a decrease in water costs with the reduction of water supply and an increase in effective operational costs, effective total operational costs and total costs, in water supply of 60% and 40%. A decrease in gross income, gross margin, net margin, profit, and profitability was observed with the 60% water supply and an increase in gross income with the 40% supply. The break-even cost values were similar among the four water offers. The cost benefit ratio in all offers was above 1. In conclusion, reducing water supply levels did not affect nutrient intake and digestibility, ingestive behavior, water and nitrogen balance, and performance. Furthermore, the proposed rearing system, regardless of the water supply level is economically feasible and can be adopted according to water availability in similar conditions of the present study.
  • LARISSA KELLEN DA CUNHA MORAIS
  • MOTHER-OFFSPRING RELATIONSHIP IN HAIR-COAT SHEEP BREEDS
  • Advisor : EDILSON PAES SARAIVA
  • Date: Jun 10, 2022
  • Time: 13:30
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  • The survival and viability of lambs are the principal objectives of the sheep production systems, which in turn, depend of the capacity of ewes to transfer sufficient amount of parental care to their offspring from birth to weaning. This thesis evaluated the maternal-offspring relationship from the first hours of birth to weaning in Santa Ines and Dorper sheep. Specifically, we would like to answer the following questions: I) Is there a difference between the Santa Inês and Dorper breeds in the maternal care and in the reactivity of lambs in the first hours after birth? II) Is there a difference between Santa Inês and Dorper breeds in the mother-offspring relationship over the lactation phase? III) Is there a difference in the magnitude of parental conflict between Santa Inês and Dorper ewes and their lambs? There were no differences between breeds in maternal and neonatal reactivity soon after delivery. Santa Ines and Dorper ewes did not show differences in latency to groom (P = 0.286) or time spent performing grooming (P = 0.074) and lambs did not differ in latency to stand (P = 0.387) and latency to suckle. (P = 0.939). Like Santa Ines and Dorper ewes, they spend the same amount of maternal care for lambs born in the first and second order and do not make suckling difficult (P = 0.4160). Throughout the lactation phase, Santa Inês ewes were more vigilant in the first two weeks after calving (P < 0.05) and Santa Inês lambs spent more time foraging close to their mother (P =0.0002). However, there were no significant effects of race on suckling attempt (P = 0.1495) or suckling rate (P = 0.6560) across observation weeks. Signs of disagreement between mother and offspring, for both breeds, are observed from the 4th 25 week of lactation. This study shows that Santa Inês and Dorper ewes spend the same amount of maternal care with their lambs and that neonatal reactivity was not affected by breed in the first hours after parturition. During lactation, however, there were racial differences in the expression of maternal care. Compared to Dorper ewes, Santa Inês ewes showed behaviors that suggest a closer association with their lambs. Furthermore, ewes and lambs disagree on the amount of maternal investment to be allocated during lactation and this conflict seems to be resolved more quickly in Santa Inês ewes.
  • ANTONIEL FLORENCIO DA CRUZ
  • CARCASS EVALUATION OF LAMBS FED DIETS BASED ON REALLOCATED SORGHUM SILAGE AND CONCENTRATE LEVELS
  • Date: May 11, 2022
  • Time: 13:30
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  • In confinement systems, maximizing animal performance is one of the essential strategies for the viability of the production system. Therefore, the inclusion of concentrates is an important strategy that favors greater fat deposition and weight gain. Thus, the objective of this work was to evaluate the inclusion of concentrate levels in diets for beef sheep on carcass characteristics. The experiment was conducted at the Scientific Research Institute (Embrapa Semiárido), located in the city of Petrolina-PE. Twenty-eight non-castrated lambs, without defined breed standard (SPRD) with average initial body weight of 18.43±2.3kg were used, distributed in a completely randomized design (DIC), with four treatments (T1: 43% of concentrated supplement; T2: 66% concentrated supplement; T3: 81% concentrated supplement; T4: 91% concentrated supplement based on dry matter) and eight replicates. You animals were confined for 60 days until slaughter. The inclusion of concentrate affected the empty carcass weight (EWC) (P=0.0493), with an increase in EBW, the measure that increased the inclusion of concentrate. For hot carcass weight (PCQ) a quadratic effect was found (P=0.0291) and there was maximization when 71.65% of concentrate was included in the diet. There were no effects of concentrate levels on neck (P=0.1979), rib (P=0.5460), shoulder (P=0.5684), loin (P=0.1046) and leg (P=0.9716), as well as gastro intestine tract weight (P=0.3220) and loin eye area (P=0.6013). There was a decreasing linear effect on hepatic glycogen, the histopathological deleterious effect on the kidneys, with an increase in epithelium thickness, keratinized portion, area of papillae and width of rumen papillae. Goblet cells increased in number in the animals up to 81% concentrate. The inclusion of concentrate contents between 60 and 72% of concentrate based on the dry matter of the feed in diets based on reallocated sorghum silage provides higher yields and carcass quality of sheep. The effects for digestibility and absorption of nutrients when considering the structures of the gastrointestinal tract were better for the inclusion between 81% and 91% of concentrate.
  • GILBERTO DE CARVALHO SOBRAL
  • Fermentative quality of corn stover silages additive with forage cactus and its use in finishing diets for sheep.
  • Date: Mar 14, 2022
  • Time: 08:30
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  • Due to the seasonal variation in forage availability throughout the year in the Brazilian semiarid region, the use of the corn stover in the form of silage becomes a viable alternative to guarantee the feeding of the herds, and when associated with forage cactus can result in the improves its nutritional value and fermentation profile. Therefore, the objective was to evaluate the effect of the inclusion of forage cactus as an additive in the preparation of corn stover silage, through the fermentative quality and performance of finishing sheep. In order to achieve the objectives, two experiments were carried out. In experiment I - levels of inclusion of forage cactus as an additive on the fermentative profile, microbial populations, aerobic stability and chemical composition of the silages were evaluated. The design was completely randomized with four treatments: 0, 10, 20 and 40% of forage cactus in natural matter, with five replications per treatment. In experiment II - the potential of three based corn stover silages added with forage cactus in diets for finishing sheep was tested. The experimental design was completely randomized with three treatments: 1) corn stover silage; 2) 90% corn stover silage with 10% forage cactus and 3) 80% corn stover silage with 20% cactus based on natural matter. The variables analyzed were: dry matter intake, water intake, productive performance, nutrient digestibility, ingestive behavior and silage losses in bags. The chemical composition of corn stover silage added with forage cactus was altered according to the level of cactus cactus. For MS (P=0.001) and EE (P=0.006) there was a decreasing linear effect, while for BP (P=0.002), MO (P=0.001) and MM (P=0.001) there was a quadratic effect . For the fermentation profile, there was a quadratic effect for pH (P=0.005) and CHOs (P=0.049). Regarding DM losses and recovery, an increasing linear statistical effect was observed for EP (P=0.0171) and a quadratic effect for PG (P=0.0350) and RMS (P=0.0057). The microbiological count showed a statistical effect, with a decreasing linear effect for BAL (P=0.030) and quadratic for ML (P=0.001). For aerobic stability, there was an increasing linear effect for the time to break stability (P=0.001). A statistical effect was observed for silage losses due to disposal after opening in laminated bags (P=0.0070). There was no statistical effect for the consumption of MS (P=0.3692), MO (P=0.0900) and CNF (P=0.0629). A significant difference was observed for water intake via a drinking fountain (P=0.0017), water intake via food (P=0.0215), total water intake (P=0.0048). The consumption of CP (P=0.0047), EE (P=0.0001) and NDF (P=0.0030) showed a significant difference, with the highest values observed for CP and EE in the treatment without the inclusion of forage cactus and for NDF with the inclusion of 20% forage cactus. There was a significant difference for rumination time (P=0.0143) and number of urination times (P=0.0102). There was a statistical effect for the rumination efficiency of MS (P=0.0205) and NDF (P=0.0055), with higher values observed in the treatment with the inclusion of 20% forage cactus. Based on the results of the two experiments, the inclusion of forage cactus improves the fermentative profile, increases aerobic stability and reduces fermentative losses and after opening the silos and its use in the sheep diet does not compromise animal performance and reduces the intake of Water. Thus, it is recommended to include 20 to 30% of forage cactus in the MN in the silage of the corn stover.
  • RAFAEL LOPES SOARES
  • USE OF REHYDRATED CORN GRAIN SILAGES FOR CONFINEMENT LAMBS
  • Date: Mar 3, 2022
  • Time: 08:00
  • Show Summary
  • Corn is among the most produced cereals in the world, the availability of starch from this grain for animal digestion is limited by several factors, including vitreousness, which is characterized as an arrangement of corn reserve proteins protecting the starch granules and making them unavailable for animal metabolism. In order to remedy this nutritional characteristic, the adoption of grain silage rehydrated with water becomes a nutritional tool to reduce its vitreous content. Among the studies carried out, there are good results for the conservation of these grains in the form of silages and satisfactory advances in the inclusion of these silages on animal performance. Studies have shown that rehydrated corn grain silage had low dry matter loss, low proteolysis and good aerobic stability. Its use in the ruminant diet promoted greater consumption of DM, CP and NFC, with considerable rates of nutrient digestibility, in addition to increased starch, DM and CP digestibility. Despite these studies, there are unsatisfactory results such as fermentation problems and reduction in lactic acid concentration in silages. In animal metabolism, some studies point to lower consumption and digestibility of nutrients. Other parameters of the fermentative characteristics of these silages have not yet been elucidated, as well as their use in lamb nutrition. The addition of additives that aim to improve their fermentation parameters should be used in order to promote better quality of these silages. Therefore, the objective was to evaluate the fermentative profile, chemical composition and aerobic stability of corn grain silages rehydrated with water or forage cactus, as well as to evaluate the animal performance and physiological parameters of lambs in feedlot fed with these silages.The use of forage cactus as a source of hydration in rehydrated corn grain silage is a viable alternative, considering that it promoted better results for fermentative profile and aerobic stability than when rehydrated with water. In the nutrition of lambs, it presented better parameters of animal performance.
  • JONATHAN MÁDSON DOS SANTOS ALMEIDA
  • DIETARY FIBER ASSOCIATED OR NOT WITH PROBIOTICS IN DIETS FOR WEAN PIGLETS
  • Date: Feb 28, 2022
  • Time: 08:00
  • Show Summary
  • This thesis work consisted of 3 experimental essays. The first presents a systematic review of studies that investigated the role of fiber in the diet of weaners and its influence on intestinal integrity and immunological markers. After using exclusion and inclusion criteria, the database consisted of 24 originals studies. It was observed that most of the articles used up to 100 animals to carry out the experiments and that the most common age for weaning was at 28 days. In several articles, it was possible to prove the effectiveness of fiber, depending on the source and level used, in the maintenance and improvement of intestinal integrity and barrier function. This research may have elucidated some important parameters when starting a field experiment. In the second trial, the objective was to carry out a a meta-analysis of the relationship between dietary fiber and the performance of piglets in the weaning phase. Through online searches in different indexing bases, a total 8360 articles were exported to the software for later selection. Graphical analyzes for to general evaluation of the data and to obtain an evalution of their consistencyand heterogeneity. Means were compared using Tukey’s test at 5% probability using the Minitab 20 program. After using exclusion and inclusion criteria, the database consisted of 57 original studies, from 18 countries and published between 1997 and 2020 in 26 different journals. Almost half of the articles were published from 2015 onwards and the most common level of NDF was recommended. Even with great variability in the data, it was possible to verify that in studies with crude fiber levels between 4 and 5%, the animals showed greater relative daily weight gain, while there was no explicit correlation between a higher level of NDF and an increase in feed intake. There was an effect (P<0.05) of the NDF on feed efficiency, in which the animals that consumed the low level had better values when compared to those that consumed the high level. A trend towards greater weight gain was observed in studies that used low and recommended levels of NDF. This study may serve as a basis for future work, based on the researched data. In the third trial, the objective was to evaluate the effects of adding lignocellulose associated or not to probiotics in diets for weaned piglets. Forty weaned piglets of the same commercial strain were used 20 castrated males and 20 females. The animals were distributed in an experimental desing in randomized blocks, with four treatments and five replications. The treatments were arranged as follows: CD: control diet composed mainly of corn and soybean meal; DF: CD + 1% of lignocellulose; PD: DC + 0.2% of probiotic; FPD: DC + 1% of lignocellulose and 0.2% os probiotic. The addition of lignocellulose and probiotic did not affect (P>0.05) performance or diarrhea incidence. There was an effect (P<0.05) of the experimental diets for the analysis of serum immunoglobulins, in which for IgG and IgM animals that consumed FPD had higher levels followed by PD, DF and CD, respectively; for IgA the consumption of the DFP diet increased the levels. Piglets that consumed diets containing lignocellulose and probiotics in association or not showed higher (P<0.05) villus height (VH) in the duodenum and ileum, and lower crypt depth and better ratio in the jejunum. The VH in the jejunum was higher for animals that consumed the FPD. Fecal butyrate profiles were higher (P<0.05) in piglets that consumed the DF and PD diets and the valerate level increased (P<0.05) from the consumption of the PD diet. In conclusion, the use of 1% of lignocellulose and 0.2 % of probiotic in association or not can be recommended because it increases serum antibodies, short-chain fatty acid profiles, in addition to improving morphometric parameters. Intestinal tract, without prejudice to performance variables, digestibility nutrients and the incidence of diarrhea.
  • LAÍLA FIONALLY ALMEIDA DE OLIVEIRA
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  • Advisor : SEVERINO GONZAGA NETO
  • Date: Feb 25, 2022
  • Time: 08:00
  • Show Summary
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  • ROMILTON FERREIRA DE BARROS JÚNIOR
  • Nutritional plans for japanese quail housed at different temperatures
  • Date: Feb 22, 2022
  • Time: 09:00
  • Show Summary
  • This study aimed to evaluate the effect of different nutritional plans and temperatures for Japanese quails from one to 39 days of age. A total of 576 one-day-old female Japanese quails (Coturnix japônica), with an initial average weight of 7.51 ± 0.75 g, were distributed in a completely randomized design in a 3 × 2 factorial scheme (three nutritional plans x two temperatures), with 12 replicates of eight birds per experimental unit. At eleven days old, the birds were transferred to two climatic chambers kept at 25º and 35ºC. The nutritional plans were: PN1 - offering two diets from 1 to 21 days and from 22 to 39 days (Silva; Costa, 2009); PN2 - offering two diets from 1 to 14 and from 15 to 39 days (Rostagno et al., 2017); PN3 - with the offer of a single diet in a period of 1 to 39 days (Silva; Costa, 2009). The variables evaluated were: performance, carcass characteristics, economic viability, in addition to curves and rates of body growth, carcass, prime cuts, feathers and edible viscera, using the Gompertz mathematical model. There were effects for planes, temperatures and interaction for performance. PN1 had lower feed intake and feed conversion, not differing from PN3 in terms of weight gain. Quails kept at 25ºC had lower feed conversion and gizzard weight, and higher feed intake and weight gain. PN3 achieved greater financial returns in both creation environments. Quails fed with PN3 also showed higher values in the curve and growth rate of body, carcass, thigh+thigh and feathers. Quails reared under heat stress (35°C) had a lower maturity rate (P<0.05). In birds kept in 25ºC, higher (P<0.05) growth rate and breast weight were observed in quails fed PN3 and those fed PN1 had higher (P<0.05) thigh+thigh weight and feather weight. Japanese quails fed with nutritional plan 3, housed at 25 ºC, have better productive performance, growth curves and financial return, without compromising carcass characteristics from one to 39 days of age, being also the most suitable nutritional plan for quails under stress by heat (35°C).
  • MARILEIDE DE SOUZA SÁ
  • Listeria monocytogenes IN PRODUCTS OF ANIMAL ORIGIN: in silico RESEARCH ON ANTIMICROBIAL RESISTANCE.
  • Date: Feb 15, 2022
  • Time: 08:30
  • Show Summary
  • Listeriosis is a disease of great impact on public health, caused by the bacterium Listeria monocytogenes due to the consumption of contaminated food, especially those from products of animal origin. The administration of antibiotics in animal production as growth promoters can trigger antimicrobial resistance, and this resistance is transferred to products of animal origin. The objective of this work was to verify if the antimicrobial resistance profiles present in Listeria monocytogenes bacteria isolated from foods of animal origin are similar to those present among strains of different origins, such as: plant, environmental and clinical foods, regardless of their region of origin. For this, the metadata of 30,148 strains of L. monocytogenes via NCBI were used. Metadata information was kept for statistical analysis. Then, the genomes were divided into six groups based on source of isolation and location among Products of Animal Origin (POA), Products of Plant Origin (POV), Animal Environments (AA), Plant Environments (AV), Animal Clinical Infections (ICA) and Human Clinical Infections (ICH). Prevalence was calculated as the ratio between each antimicrobial resistance gene and the total number of genomes evaluated in each group. These data were used to build the prevalence heat map. 45 resistance genes were identified, 15 from Clinical Animal, 24 from Clinical Human, 7 from Animal Environment, 19 from Plant Environment, 11 from Animal Products and 34 from Plant Origin. The tetM and fosX resistance genes had a common prevalence in all regions. The prevalence map generated two distinct clusters of genomic sequences obtained according to the resistance profile, in which bacteria that were associated with the animal and plant food chain, including food and the environment, were grouped in Cluster 1, while those from clinical sources animals and humans were grouped into Cluster 2. The main resistance characteristics observed were the high frequency of InuG in the genomes of L. monocytogenes from Animal Environment, Plant Environment and Products of Plant Origin, the high frequencies of mefA and aph(3' ) – Illa in genomic sequences of plant origin (environment and food, respectively), and the high frequencies of tetM and tetL in L. monocytogenes of animal origin. Resistance of human clinical isolates was characterized by high frequencies of tetS and fexA genes, while animal clinical isolates showed high abundance of vgaA and aadD1. Thus, the antimicrobial resistance genes of the bacterium L. monocytogenes isolated from products of animal origin were similar to those found in strains of other origins, making it necessary to support an epidemiological surveillance plan in the country regarding the occurrence of this bacterium.
  • FRANCISCA FERNANDA DA SILVA ROBERTO
  • Sheep finishing in Brachiaria spp. supplemented with levels of amireia to replace soybean meal.
  • Date: Jan 28, 2022
  • Time: 09:00
  • Show Summary
  • The aim was to evaluate the effect of replacing soybean meal with extruded urea (Amireia® 200S) at increasing levels (0; 6; 12; 18 and 24 g 100 kg-1 of live weight (LW) in the supplementation of beef lambs) finished on Brachiaria spp. pastures, on the structural and nutritive characteristics of the forage; nutritional value of the supplement; dry matter intake (DMI); ingestive behavior; performance; parasitological aspects and their interactions in the host-pasture-soil components; carcass, non-carcass components and meat quality. Sixty Texel crossbred lambs, with an initial weight of 20.7±0.87 kg and 2.5±0.7 months of age, were evaluated. 6 animals of each treatment were used, and for the slaughter and evaluations of carcass and derivatives, 9 animals were used per treatment. For the variables, forage mass, forage accumulation rate and structural characteristics of the pasture there was no treatment effect o (P>0.05). The forage DMI in percentage of LW and metabolic weight were adjusted to the quadratic equation, in which it was estimated that the minimum DMI in relation to LW (1.5%) and by the metabolic weight (55.6 g MS/kg^0,75) would be found at levels 10 and 8.3 g 100 kg-1 LW of extruded urea, respectively. Animals that received 18 g 100 kg-1 LW had the highest average daily gain. Grazing time showed a decreasing linear effect, supplement consumption increasing linear effect, as well as the biting rate as a function of the levels. Animals from treatments 6 and 18 g 100 kg-1 LW spent more time ruminating. The search for water was higher for animals that received the highest level of replacement, and lower for animals treated with 18 g 100 kg-1 LW. Extruded urea levels of up to 18 g 100 kg-1 LW maintained animal performance even when both groups had egg counts per gram of faeces above 1200, with no apparent impairment of metabolic status or welfare. There was less dispersion of gastrointestinal nematode larvae in the environment (pasture and soil) when increasing the level of replacement of soybean meal by extruded urea; and clayey soil did not prove to be a good reservoir for gastrointestinal nematode larvae. A significant effect (P<0.05) of extruded urea levels was observed in lamb supplementation for slaughter weight; body condition score at slaughter; empty body weight; hot carcass weight; finishing; loin eye area and subcutaneous fat thickness. For non-carcass components in general, no effect of diet was observed (P>0.05). As for the quality of the meat, the variables, pH; exudation loss; cooking loss; L*, a* and b* on muscle and fat 24 hours post-slaughter there was no significant effect (P>0.05) of the supplement. In the chemical composition of the meat, there was an interaction between level x sex for ether extract and crude protein, the meat of the females of the treatment without extruded urea had a higher content of ether extract and the meat of the males of the treatments with 12 g and 24 g 100 kg-1 LW higher crude protein contents. The replacement of soybean meal with extruded urea in the diet of beef sheep can be provided up to the level of 18 g 100 kg-1 LW, without negatively compromising consumption and ingestive behavior, animal performance, most carcass characteristics, components non-carcass, meat quality and chemical composition; assists in the resilience of hosts to gastrointestinal nematodes and without causing ammonia intoxication or any visible disturbance.
2021
Description
  • TARSYS NOAN SILVA VERISSIMO
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  • Advisor : EDILSON PAES SARAIVA
  • Date: Dec 29, 2021
  • Time: 08:30
  • Show Summary
  • The migration of dogs to regions with climates totally different from their place of origin, as well as the phenotypic changes that occur throughout evolution, are starting points for questions to be raised about the tolerance that these animals have to the climatic conditions where they live. . Some phenotypes explored over time in certain breeds are related to improved health, while many others negatively affect the well-being of domestic dogs, making them very susceptible to high air temperatures. The sensitivity to heat that dogs have is linked to some factors, such as limitation in thermolysis, as they use respiratory evaporation as the only efficient mechanism for thermal exchanges in situations of heat stress. In addition, the common practice of physical activities in conjunction with exposure to solar radiation or humid environments can increase the risk of hyperthermia in domestic dogs, in addition to the possibility of developing respiratory alkalosis, hypoglycemia, dehydration, irreversible neurological damage and even even death. Thus, it is important to know the thermal exchange mechanisms that contribute to the thermoregulation of dogs, as well as the thermal balance models already developed, so that strategies are established to reduce the negative effects of thermal environments that are unsuitable for the species and improve the well-being of domestic dogs raised in tropical climate regions.
  • PRISCYLLA CARVALHO VASCONCELOS
  • Comparative genomic study of Staphylococcus spp. associated with caprine subclinical mastitis in Paraíba
  • Advisor : CELSO JOSE BRUNO DE OLIVEIRA
  • Date: Dec 17, 2021
  • Time: 08:00
  • Show Summary
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  • ROSEVÂNIA VELOSO BARBOSA
  • FIBER BARS FORMULATED WITH AGROINDUSTRIAL FRUIT RESIDUES FOR PET RABBITS
  • Date: Dec 10, 2021
  • Time: 09:00
  • Show Summary
  • The population of pets has grown in families' homes, and unconventional animals such as rabbits have gained ground both for their beauty and for their docility, in addition to being the small animal. Rabbits are animals that need dietary fiber to meet their nutritional needs, physiological and behavioral aspects. In this context, the objective was to evaluate the use of dietary fiber bars formulated with different agribusiness fruit residues for Pet rabbits. For that, 24 animals of both sexes with 50 days of age were used and distributed in a completely randomized design divided into four treatments and six replications. The treatments consisted of offering feed plus fiber bar, as follows: RC - Commercial feed; RAC Commercial feed + Fiber bar formulated with acerola residue; RCA - Commercial ration + fiber bar formulated with guava residue and RGO - Commercial ration + fiber bar formulated with guava residue. The productive performance was analyzed; ration digestibility, animal behavior, SCFA profile and wear of animal teeth as a function of bar consumption. The results showed that the consumption of bars did not affect (P>0.05) the productive parameters, as well as the digestibility of the diets in the evaluated periods. The short-chain fatty acid profile of the feces was not influenced by the consumption of fiber bars, except for the acetic acid concentration which was reduced (P<0.05) in the feces of rabbits fed with the bars. Regarding tooth wear, the consumption of fiber bars, regardless of the residue, reduced (P<0.05) the size of the upper teeth, not affecting the lower teeth in the entire period evaluated. It is concluded that the offer of fiber bars formulated with acerola, cajá and guava residues together with the feed helps in tooth wear, without affecting the performance and digestibility of the feed for Lion Head rabbits.
  • IARA TAMIRES RODRIGUES CAVALCANTE
  • CARCASS TRAITS AND QUALITY OF LAMB MEAT FED WITH SILAGE BASED ON SPINELESS CACTUS AND COTTON PIE
  • Date: Aug 30, 2021
  • Time: 14:00
  • Show Summary
  • The study aimed to evaluate the carcass traits, meat quality and the yield of non-carcass constituents of lambs fed total mixed ration on spineless cactus and cotton cake. Thirty-two crossbred Dorper lambs with initial age of 120 days and average initial live weight of 17±1.5 kg were used. After 60 days of confinement, the lambs were evaluated biometrically, weighed, slaughtered, eviscerated and the carcasses obtained were measured morphometrically. The experiment was carried out in a completely randomized design with four treatments (0, 20, 25 and 30% inclusion of cotton cake) and eight replications. Data for each treatment were compared using orthogonal contrasts, which when significant by Dunnett's test, were submitted to regression analysis at 5% probability. The biometric variables were subjected to principal component analysis, Pearson correlations and used in the development of the carcass traits prediction equation through regression analysis at 5% probability. The morphometric and biometrics data were submitted to canonical discriminant analysis to identify the variables that best discriminate the treatments and build a discriminant function that represents the differences between the treatments. There was no effect (P>0.05) of treatments on carcass characteristics, weight and yield of commercial cuts, weight of edible and inedible organs, tissue composition, pH, temperature, color, cooking losses, moisture and content of protein from the meat of the lambs. The equality of results may be associated with the fact that the diets are isoprotein and isoenergetic, but the diet containing 25% cotton cake proved to be more economically viable. Among the biometric variables studied by multivariate analysis, 6 body measurements fit (P<0.05) to the prediction equations of slaughter weight, empty body weight, hot carcass weight, cold carcass weight, hot carcass yield, cold carcass yield, ribeye area and edible viscera weight, suggesting that producers who do not have a scale and want to use the experimental diet can predict meat production from biometric measurements using the equations generated in this study. The variables with greater discriminatory power between treatments, selected based on standardized canonical coefficients were chest depth (with greater discriminatory power by the stepwise method), withers height and leg width. The first two canonical variables were sufficient to explain 73% and 20% of the total data variation. The inclusion of up to 30% of cotton cake as a replacement for buffel hay in the spineless cactus silage can be used in the termination of lambs in harming the production of meat in confinement.
  • DANILLO MARTE PEREIRA
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  • Date: Aug 20, 2021
  • Time: 08:00
  • Show Summary
  • This study involved three experiments. In the first, the objective was to identify the optimal level of ideal forage cactus in the mixture that provides a good microbiological profile, as well as reducing losses during the ensiling process, optimizing dry matter recovery and aerobic stability of mixed silages. The experimental design consisted of a completely randomized design, consisting of five treatments and four replications. The treatments were represented by the inclusion of spineless cactus in the forage sorghum silage: 0; 25; 50; 75 and 100% of forage cactos in the natural matter of the mixture. There was an increasing quadratic effect (P < 0.05) on lactic acid bacteria counts (LAB) and dry matter recovery (DMR), estimating maximum values at levels of 51.8 and 33.5% of forage cactus in the silage. However, there was a decreasing quadratic effect (P < 0.05) for gas losses (GL), with the lowest value being estimated at the level of 37.9% of forage cactus in silage. There was an increasing linear effect (P < 0.05) for effluent losses (EL), time in which the silages reached the maximum temperature (TMT), aerobic stability (AS) of the silages and in the levels of ether extract (EE) and non-fibrous carbohydrate (NFC). However, there was a decreasing linear effect (P < 0.05) on mold count, maximum silage temperature (MT), dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) contents of the silages mixed. When using the forage cactus in the confection of mixed silages with sorghum, it is recommended the 50% level of forage cactus, optimizing the recovery of dry matter and aerobic stability, without affecting the nutritive value of the silage. The second experiment aimed to evaluate the effects of forage cactus as humidifier additive on fermentative, microbiological, aerobic stability, chemical composition and In situ ruminal degradability of corn grain silage at different opening times. The experimental design consisted of a completely randomized design in a 4 × 3 factorial scheme was used, consisting of four levels of dry matter (DM) (50; 60; 70 and 80% DM) and three opening periods (30; 60 and 120 days after ensiling). There was an interaction effect (P < 0.05) between DM levels of maize grain silage rehydrated with forage cactus and the opening periods on the silage yeast population, effluent losses (EL), gas losses (GL), dry matter recovery (DMR), aerobic stability (AS) and forage cactus levels on lactic acid bacteria (LAB) populations, molds and yeasts, after the AS test. The DM In situ degradability of the ensiled mass increased after the ensiling process at all levels and opening periods evaluated. When using forage cactus as a humidifying additive for corn grain, the 60% DM level is recommended when the permanence time of the ensiled mass in the silo is equal to or less than 120 days and 70% DM when it is 120 days after the silage. In the third experiment, the objective was to evaluate the fermentative characteristics of silage total mixed ration based on forage cactus and cotton cake and its effect on the productive performance and ingestive behavior of sheep in confinement. The study was divided into two experimental trials, both distributed in a completely randomized experimental design. The first experimental trial evaluated the fermentative quality of silages total mixed ration (TMR), with five repetitions per treatment, and the second evaluated the productive performance and ingestive behavior of the sheep, with eight repetitions per treatment, with the treatments represented by the pie proportions of cotton cake in the TMR (0; 20; 25 and 30% cotton cake, based on dry matter). There was a significant difference (P < 0.05) between the TMR for yeast population, pH, gas losses (GL), efluente losses (EL), dry matter recovery (DMR), ether extract intake (EEI), dry matter digestibility (DMD), organic matter (OMD), crude protein (CPD), ether extract (EED), neutral detergent fiber (NDFD), non-fibrous carbohydrate (NFCD), total digestible nutrients (TDN) and for all ingestive behavior variables, except for feeding. Thus, forage cactus ensures the fermentative and hygienic quality of the silages in the form of feed, regardless of the proportions of cotton cake. Therefore, based on the productive performance and ingestive behavior of Dorper sheep feedlot, it is recommended silage as feed with 30% cotton cake.
  • GUILHERME MEDEIROS LEITE
  • Detoxified castor bean bran as a protein additive in sugarcane silage
  • Advisor : EDSON MAURO SANTOS
  • Date: Aug 6, 2021
  • Time: 09:00
  • Show Summary
  • The aim of this study was to evaluate the influence of castor bean meal inclusion levels with two particle sizes on chemical composition, microbial populations, fermentation profile, losses during ensiling and aerobic stability of sugarcane silages. A completely randomized experimental design was used, in a 4x2x2 factorial scheme, with four levels of inclusion of castor bean meal based on natural matter (0, 5, 10 and 20%), two particle sizes (<0.5mm and >0, 5mm) and two opening times, 30 and 100 days, with four repetitions. The experimental silos contained Bunsen valves to quantify losses. The sugarcane was chopped at 2cm, followed by mixing with the castor bean meal. The material was compacted until reaching a density of 600 kg/m3 . There was an increasing linear effect for crude protein on the inclusion levels of castor bean meal, in both granulometries and opening periods (P= 0.0001), with a mean of 21.36 and 19.54 g Kg-1 of DM. There was a quadratic effect for mold at the 100-day opening for the inclusion levels (P=0.0001), with an estimated minimum count at the inclusion level of 10.82%. An increasing linear effect was observed for the buffer capacity at the levels in the fine granulometry (P= 0.0196) of the 30-day opening. For the maximum temperature reached by the silo, there was a quadratic effect (P= 0.0011) for the inclusion levels, observing the lowest maximum temperature at the inclusion level of 13.69%. When using detoxified castor bean meal as a protein additive in sugarcane ensilage, it is recommended the 20% inclusion level for opening at 100 days, regardless of the particle size used.
  • ALICE DA ROCHA SILVA
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  • Advisor : ARIOSVALDO NUNES DE MEDEIROS
  • Date: Jul 30, 2021
  • Time: 09:00
  • Show Summary
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  • DIEGO DE SOUSA VIEIRA
  • MICROBIOLOGICAL COMPOSITION OF FORAGE PALM AND BUFFEL GRASS DIETS AND ITS RELATIONSHIP TO NUTRITIONAL DISORDERS IN SHEEP
  • Date: Jul 30, 2021
  • Time: 08:30
  • Show Summary
  • The water deficit existing in arid and semi-arid regions makes forage cactus stand out as an alternative in ruminant feeding, but this, as it has a low level of neutral detergent fiber (NDF), must be supplied to animals in conjunction with sources of fiber such as buffel grass hay. The objective of this work was to evaluate the inclusion of buffel grass hay levels in diets based on cactus forage on metabolic disorders in confined sheep. The experiment was conducted on a private property, located in the city of São José dos Cordeiros-PB. Four diets constituted with different percentages of buffel grass hay were tested. The diets were composed of forage cactus, buffel grass hay and concentrate, and the treatments were represented by different levels of hay in the dry matter of the ration: T1: 7.5% buffel grass hay; T2: 15% buffel grass hay; T3: 30% buffel grass hay and; T4:45% buffel grass hay based on dry matter. The variables analyzed were: Escherichia coli and enterobacteria counts; fecal score; dry matter consumption; physiological parameters; blood count. The interaction effect of collection period and buffel grass hay levels was observed on the counts of Enterobacteria (P<0.0001) and E. Coli (P=0.0043) in leftover food and animal feces. The levels of buffel grass hay had a quadratic effect on dry matter intake (P=0.0101) with maximization of the DMI at the level of 25.3% of buffel grass hay. There was no interaction effect (P=0.9311) on the fecal score of the animals. However, there was a significant difference (P=0.0034) in the levels of buffel grass hay on this variable. There were significant changes (P<0.0001) in the blood parameters of sheep. Levels below 15% of buffel grass hay based on dry matter, in diets based on cactus forage, provides greater contamination of the diet and animal feces by enterobacteria, such as Escherichia coli, negatively modifying the blood and physiological parameters and increased incidence of diarrhea in confined sheep.
  • DAIANE GONÇALVES DOS SANTOS
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  • Date: Jul 29, 2021
  • Time: 08:30
  • Show Summary
  • The aim of this study was to evaluate the bacterial community dynamics of different proportions of forage cactus and buffel grass hay as a function of the period of exposure to air. The experimental design used was completely randomized, in a 5 x 5 factorial arrangement (five levels of palm and buffel grass hay x five times of exposure to air), with five replications. The treatments consisted of forage cactus levels, ranging from 20 to 100% in natural matter, evaluated at times 0, 3, 6, 12 and 24 hours. The variables analyzed were: internal and surface temperature; chemical composition; Escherichia coli population and bacterial community by 16S ribosomal rRNA gene metataxonomy. There was an interaction between the levels of palm in the diet and the time of exposure to air (P<0.001) for internal and surface temperature, pH and population of Escherichia coli. It was found that the lower the level of palm, the higher the internal and surface temperature of forages, reaching the peak temperature after 12.19 and 11.87 hours of exposure to air, respectively. In all treatments, the pH was considered as having low acidity, between 5.70 and 7.38. The peak of E. coli population growth was after 16.06 hours of exposure to air. For chemical composition, there was interaction (P<0.001) of palm levels and time of exposure to air for dry matter, crude protein, buffer capacity, soluble carbohydrates and ammonia nitrogen. The time of exposure to air significantly influenced (P<0.001) the values of organic matter, mineral matter and ether extract, for the values of neutral detergent fiber it observed a significant effect (P<0.001) for the levels of palm. An increase in microbial richness and uniformity of all treatments was noted after six hours. The most abundant genera were Weissella, Lactobacillus, Bacteroides, Pseudomonas, Sphingobacterium and Sphingomonas. A higher microbial quality was observed in the diet with 100% palm, with a predominance of Weissella, Lactobacillus and Leuconostoc. With 80% of buffel grass hay there was an apparent abundance of Pseudomonas, Sphingomonas and Sphingobacterium. Thus, it is concluded that forage cactus in combination with buffel grass hay increases the proliferation of microorganisms with pathogenic potential, when exposed to air for periods longer than 6 hours, negatively influencing the microbiological quality and chemical composition of forage.
  • JORGE HENRIQUE DA SILVA COSTA
  • HERBACEOUS-SUBARBUST STRATUM IN CAATINGAAREAS PAINTED BY GOATS ASSOCIATED WITH PHOSPHATE SOIL FERTILIZATION
  • Date: Jul 15, 2021
  • Time: 09:00
  • Show Summary
  • The Caatinga has a diversity of native species and high availability of plants of forage interest, however the structure and plant production vary according to environmental conditions and management. In order to evaluate the Caatinga under different doses of phosphate fertilization, from May 2019 to June 2020, an experiment was conducted at the Experimental Station of São João do Cariri, belonging to the Center for Agricultural Sciences of UFPB, in the city of São João do Cariri. The total experimental area used was 9.6 hectares, divided into three sub-areas of 3.2 hectares, which were submitted for 10 years (2007 to 2017) to different grazing intensities by goats: 10; 5 and; area without animals. During the experimental period there were no animals in the areas. For evaluation, each area was subjected to 4 treatments (control, 30, 60 and 90 kg of P2O5/há), these presented in five transects, three transects that received phosphate fertilization with 400m² (2m wide x 200m long) and two transects (control) with shorter length and minimum distance of 5 m so as not to be influenced by fertilization, where 10 samples were collected in each treatment, totaling 120 samples each month. After separation and identification, the species that showed greater availability (Aristida adscensionis, Diodia teres, Malvaceas and the Pool) were evaluated for frequency, dominance and forage mass. Fertilization promoted an increase in forage mass production in areas of Caatinga do Cariri in Paraíba, with emphasis on the dose of 90 kg of P2O5/há in 2020. At the month of April 2020 presented the highest mass production. Aristida had the highest frequency and dominance among species, while Diodia had the lowest relative frequency value in area 2 with 16.28%. For dominance, Aristida showed superiority among species, with an average of 30.50% in the evaluated areas. Assessing the relationship between year and species, the first two main components contributed with 89.1% to data variation. It was observed, greater availability of Aristida in area 2 in the year 2020 with an average of 58.293 g/m². Considering the relationship between year, area and P2O5 dose, the control treatment for the year 2019 had a lower forage mass value. In this sense, the doses of phosphate fertilization, together with high precipitation, promoted, regardless of the use of the area, a greater production of forage mass.
  • LILIANE PEREIRA SANTANA
  • MICROBIAL INOCULANT AND COTTON PIE IN MILLET SILAGE IN DIFFERENT FERMENTATION PERIODS
  • Date: Mar 9, 2021
  • Time: 09:00
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  • An experiment was carried out in the Forage sector of the Department of Zootechnics of the Agricultural Sciences Center of the Federal University of Paraíba, in Areia with the objective of evaluating the result of the addition of microbial inoculant and cotton cake on the fermentation profile, microbial populations, stability aerobic, chemical composition and losses in millet silages. The experimental design used was completely randomized, in a 4 × 2 factorial scheme, with the treatments being: 1. Control (Millet); 2. Millet + 10% cotton cake (based on natural material); 3.Millet + Weissella cibaria - heterofermentative lactic bacteria; 4. Millet + cotton cake and Weissella cibaria (Combo) and two opening periods (30 and 90 days after silage) and four repetitions, totaling 32 experimental units. Quantification of microbial populations, fermentation profile, gas losses were evaluated and effluents in silage, dry matter recovery, chemical composition and aerobic stability. The silages containing cotton cake had a greater recovery of dry matter (P = 0.0002). There was an effect of the opening on the pH of the silages (p <0.0001). At 90 days of opening, in cake silages, yeast and mold counts were less than 1.00 log UFC g-1 of silage. There was an effect of the opening in the counts of BAL, mold and yeast (p <0.0001) and silages opened at 90 days had lower counts. There was also an effect of the opening on aerobic stability (p <0.0001) in which silages opened at 90 days, there was the greatest stability. The addition of cotton cake provided an increase in the contents of DM, MM, EE, and PB (p <0.0001) and a decrease in the contents of NDF and FDA (p <0.0001), when compared to control silages (only millet). ) and inoculated with Weissellacibaria. The losses due to effluents were much lower in silages containing cotton cake. The use of cotton cake in millet silage proved to be efficient in decreasing silage losses, improving the fermentative profile and nutritional value, however, it did not influence the aerobic stability of millet silage, as well as Weissella cibaria.
  • NATALÍ ESTEVÃO DA CRUZ
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  • Advisor : JOSE HUMBERTO VILAR DA SILVA
  • Date: Feb 26, 2021
  • Time: 14:00
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  • LEANDRO PEREIRA DE OLIVEIRA
  • ANATOMICAL, CHEMICAL AND NUTRITIONAL CHARACTERIZATION OF Poincianella pyramidalis (Tul.) L. P. Queiroz ASSOCIATED WITH ITS PHENOPHASE
  • Date: Feb 26, 2021
  • Time: 09:00
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  • The Poincianella pyramidalis (Tul.) LP Queiroz is a tree whose foliage is consumed by domestic ruminants in the dry season. local livestock efficiency. Despite the increase in research aimed at sustainable animal production in the Caatinga, the real potential for livestock production is still not known, perhaps due to the lack of knowledge of how native vegetation species behave. Thus, the objective of this work is to characterize the potential use of P. pyramidalis in animal production, from its anato-morphological, histochemical and nutritional characteristics. Leaf samples were collected in different phenophases, comprising young leaves, expanded leaves and senescent leaves (litter) for anatomical, morphological, phytochemical and bromatological analyses. The leaflets of P. pyramidalis present a distinct anatomical pattern and differences in the histolocation of the groups of secondary metabolites according to the stages of development. The anatomical characteristics of the leaves favor rumen degradability, but the presence of secondary metabolites may limit its use. The phytochemical study reveals the presence of groups of secondary metabolites of pharmaceutical interest and chemical characteristics that are relevant in animal production and welfare. The young leaf and the senescent leaf present better chemical-bromatological composition. The senescent leaf stands out for containing the highest carbohydrate content possible to be used by ruminal microorganisms and reflects in greater availability of energy for the animal. P. pyramidalis is an important food source for animals in the semiarid region.
  • MIKAEL LEAL CABRAL MENEZES DE AMORIM
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  • Advisor : ROBERTO GERMANO COSTA
  • Date: Feb 26, 2021
  • Time: 08:00
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  • THAMARA FERREIRA DA ROCHA
  • CHARACTERIZATION OF THE GUT MICROBIOME OF Apis mellifera BEES FROM CAATINGA AND ATLANTIC FOREST BIOMES IN PARAÍBA STATE
  • Advisor : CELSO JOSE BRUNO DE OLIVEIRA
  • Date: Feb 25, 2021
  • Time: 14:00
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  • The composition of the intestinal microbiota can shift by several host´s intrinsic and extrinsic factors such as diet, physiological status and genetic profile. The present study aimed to characterize the differences in the intestinal microbiota of Apis mellifera bees from two biomes (Atlantic Forest and Caatinga) by means of 16S rRNA metagenomics. Amplicons targeting the V3-V4 regionsof the 16S rRNA was sequenced using Illumina hightroughput sequencing and data processing by downstream bioinformatics. Although the seven most abundant microbial taxa were present in the gut of bees from both biomes, Apibacter was significantly less abundant in the gut of bees from Caatinga compared with Atlantic Forest. The findings revealed that bees exposed to different types of biomas showed significant differences in the abundances of gut bacteria, even though the microbial variability was relatively low.
  • ALITON NUNES DA SILVA
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  • Advisor : JOSE HUMBERTO VILAR DA SILVA
  • Date: Feb 25, 2021
  • Time: 09:00
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  • IVA CARLA DE BARROS AYRES
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  • Date: Feb 25, 2021
  • Time: 09:00
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  • Changes in intestinal microbiome of birds can be promoted through the diet, so the constituents of the diet deserve attention about their effects on the intestinal ecosystem, among them fiber and the use of enzymes have a capacity to modulate the microbiota. The objective of this study is to analyze the modulation of the microbiota of European quails diets based on corn, soybean meal and wheat bran with or without the use of xylanase and phytase.224 European quails were used, distributed in a completely randomized design with eight treatments, seven replications with four birds per experimental unit. The treatments consisted of: Diet 1 based on corn + soybean F. (T1); Diet 1+ xylanase (T2); Diet 1 + phytase (T3); Diet 1 + phytase + xylanase (T4); Diet 2 based on wheat+ soybean F. (T5); Diet 2 + xylanase (T6); Diet 2+ phytase (T7); Diet 2+ phytase+ xylanase (T8). At 42 days, ten birds from each treatment were slaughtered to collect the intestinal contents after collection, a pool was performed, where the samples were identified, frozen and stored in a falcon tube and sent to the Neoprospecta Microbiome Technologies laboratory for analysis of the microbiota.The microbial profile was affected by dietary changes and enzyme supplementation, in both diets without enzyme supplementation there was a greater predominance of the genera Streptococcus and Lactobacillus and the species Lactobacillus agilis, Streptococcus macedonicus and Lactobacillus salivarius, however, the use of xylanase in the maize diet had a predominance of S. macedonicus followed by L. agilis and L. salivarius, however in the wheat bran diet there was a predominance of L. agilis and L. salivarius species, the use of phytase in the Maize diet showed greater dominance of L. agilis, when replacing corn with wheat bran, there was dominance of S. macedinicus, however, the association of the two enzymes provided a greater amount of L. salivarius and L. agilis with different proportions between the Maize or Wheat bran based diets. The use of wheat bran with the use of phytase and xylanase modulates the European quail microbiota, with an abundance of specific bacteria (L. agilis, L. salivarius and S. macedonicus) and dominance of lactic acid bacterias.
  • MARILANIA DA SILVA SANTOS
  • DIAZOTHROPHIC BACTERIA IN FABACEANS OF CAATINGA WITH POTENTIAL FOR INOCULATION IN SORGHUM CULTIVATED UNDER HYDRIC DÉFICIT
  • Date: Feb 25, 2021
  • Time: 09:00
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  • Rhizobia make biological nitrogen fixation (BNF) in symbiosis with leguminous plants. Mimosa tenuiflora, Mimosa caesalpiniifolia and Desmanthus pernambucanus are some of the native fabáeas of the Semi-arid region that fix N and still have characteristics of adaptability and resistance to the variability of the edaphoclimatic conditions of that region. In grasses these bacteria are relevant in the free life state, using the supply of carbohydrates from the environment to fix nitrogen (N). Studies have shown that the inoculation of rhizobia can promote the growth of grasses, due to the fact that these bacteria produce plant hormones, control phytopathogenic agents, among others. Grasses grown in the semiarid, such as sorghum, may not produce good production, so it is essential to find ways to mitigate this effect of drought and low amount of N in organic form in the soil. This study involved two experiments. The first aimed to evaluate the growth and morphogenic characteristics of forage sorghum inoculated with rhizobia native to the Caatinga under contrasting water conditions. For this purpose, a completely randomized design with a 6 x 3 factorial arrangement was adopted. The inoculation was performed with 5 isolates (MTBV77, MTP78, DPP1, MTBV12 and MCLR34) from rhizobia of forage species (Mimosa tenuiflora, Desmanthus pernambucanus and Mimosa caesalpiniifolia) and soil of the Semi-arid region, and another control subjected to three water conditions, with 4 repetitions. The growth, morphogenic and structural characteristics of sorghum were subjected to analysis of variance and regression by the statistical program ASSISTAT and main components by the program R. There was an interaction between the factors evaluated for most of the variables, except for the number of live leaves and the leaves. growth characteristics. The evaluated variables of sorghum showed higher values when subjected to inoculations with isolates compared to the control except for root dry matter and total nitrogen. The positive effect of these characteristics increased linearly according to the progress of the normal water condition. The native isolates of the forage species promote growth and alter the morphogenic characteristics of sorghum under contrasting water conditions, the isolates MTBV77, MTP78, DPP1 and mainly MTBV12 demonstrate potential for the production of promising biological inoculants. In the second experiment, the objective was to evaluate the growth promotion and morphogenic characteristics of sorghum inoculated with native rhizobia associated with doses of N. The experimental design was completely randomized with a 6 x 4 double factorial arrangement, 5 of which were isolated from rhizobia species. native forages, plus a control treatment subjected to doses of N (0, 40, 70 and 100kg of N / ha-1). Morphogenic and structural characteristics and growth promotion were evaluated. The values of the sorghum variables inoculated with the native rhizobia isolates, show linear growth with the doses of N. Among the inoculants evaluated, some presented higher NT (MTBV12, DPP1 and MTP78), higher MSPA (MTP78, MTBV12 and MTBV77) and MSR (MCLR34). Indicating the promotion of growth of these rhizobia. Nitrogen fertilization cannot be dispensed with entirely, but these inoculants can allow savings in the use of nitrogen fertilizers, reducing production costs and contributing to sustainable management.
  • DANIEL SILVA SANTOS
  • INTESTINAL MORPHOLOGY OF EUROPEAN QUAIL (COTURNIX COTURNIX) FED WITH GLUTAMINE ASSOCIATED WITH GLUTAMIC ACID SUBMITTED TO THERMAL STRESS
  • Advisor : PATRICIA EMILIA NAVES GIVISIEZ
  • Date: Feb 25, 2021
  • Time: 08:30
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  • The poultry industry is considered an extremely important sector in the production of food for the world population. Quail production is one branch that has shown to be economically interesting due to its high productive potential, both for industrial production and for small producers or family farmers. This study evaluated how thermal stress affects the intestinal morphology of European quails and whether dietary supplementation with glutamine associated with glutamic acid can minimize the deleterious effects of thermal stress on the intestinal morphology of birds during their production cycle. In the first experiment, the intestinal morphology of European quails supplemented with glutamine associated with glutamic acid (Gln + Glu) was evaluated, submitted or not to cold thermal stress from 1 to 21 days of age. In the second experiment, the influence of dietary supplementation of glutamine associated with glutamic acid (Gln + Glu) on the intestinal morphometry of European quails subjected or not to heat stress from 22 to 42 days of age was evaluated. In both experiments, 320 non-sexed European quails were used, distributed in a completely randomized design (DIC), according to a 2 x 2 factorial scheme, with two thermal conditions and two diets (basal diet and diet supplemented with 1% Gln + Glu), with eight replicates and 10 birds per experimental unit. The variables analyzed were villus height, villus width, crypt depth, villus/crypt ratio and absorption area. The data were submitted to analysis of variance, and the means were compared using the Tukey test. In the first experiment, it was concluded that cold stress in the early life of European quails can be considered a factor that negatively affects the morphological development and some morphometric parameters of the small intestine. In the second experiment, supplementation positively affected the crypt depth of the duodenum and the villus/crypt ratio of the jejunum of heat-stressed European quails.
  • ANDREZZA KYARELLE BEZERRA DE MOURA
  • STOCK INDICATORS AND EFFICIENCY IN THE USE OF NUTRIENTS AND WATER IN BIOSSALINE SYSTEMS IN PALM FORAGE, SORGHUM AND MILLET
  • Date: Feb 23, 2021
  • Time: 08:00
  • Show Summary
  • The objective was to evaluate stocks and efficiency of the use of nutrients and water from the forage palm clone Mexican Elephant Ear (Opuntia stricta (Haw.) Haw.), BRS Ponta Negra sorghum (Sorghum bicolor) and ADR 300 millet (Pennisetum glaucum) grown in biosaline systems, with different irrigation depths and doses of organic matter. The experiment was designed in randomized blocks with four treatments arranged in a 4 x 4 factorial scheme with four repetitions for forage palm and three repetitions for sorghum and millet. The first factor was made up of four irrigation depths: 0% (control) rainfed condition; 12%; 20%; and 28% ETo, where ETo% is the calculated reference evapotranspiration, which added to the rainfall for the forage palm; four irrigation depths for sorghum and millet: 25%; 50%; 75%; and 100% ETo in the 1st and 2nd cycles; and the second factor was composed of four levels of organic matter: 0, 15, 30, and 45 t / ha (tanned bovine manure). The composition, stock, efficiency of nutrient use, crop water productivity and economic productivity of water from forage palm and sorghum showed variation by ETo%, MO and / or, by their interaction (P <0.05). Millet, on the other hand, was influenced by ETo%, and by the interaction between water depth and organic matter (P <0.05). In the isolated observation of organic matter, there was no significant effect (P> 0.05) for the variables in the millet culture. The application of ETo% in the studied crops (palm, sorghum and millet) contributed to the accumulation of salts in the soil, influencing the production of green matter. The increase in OM contributed to the production of forage palm at the ages of 6, 9, 12 and 15 months, of sorghum and millet in both cycles. In conditions of low water availability and / or with low quality water, bovine manure as a source of organic matter helped the water use by crops. Thus, the use of saline water for the irrigation of forage palm associated with increasing levels of organic matter had an influence on the mineral composition, however with respect to the stock of minerals they increased significantly. For sorghum, combinations of water layers with organic matter levels increased the mineral composition, however with respect to the mineral stock, there was an increase with the application of OM observed in isolation. As for millet, the use of saline water for irrigation showed little variation, which can be seen in the tolerance of the crop to salt stress, both for composition and mineral stock and for water efficiency indicators. Irrigation with saline water for water and nutrient use efficiency indicators was more effective in rainfed conditions for palm and sorghum at 25% ETo. For the economic productivity of water from forage palm, the financial return for each m3 of water was more effective with the combinations of water depths with the dose of 30t / ha at the ages of 6 and 9 months, and for the other ages at 45t /there is. Sorghum was more efficient with the combination of the ETo25% water slide with the 45t / ha dose in both cycle 1 and cycle 2. For millet the return was greater in the combination of the ETo25% water slide with the dose of 0t / ha in cycle 1 and with 45t / ha in cycle 2.
  • AYRTON FERNANDES DE OLIVEIRA BESSA
  • Genetic parameter estimates for traits of economic importance in Brahman cattle
  • Date: Feb 10, 2021
  • Time: 09:00
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  • As the Brazilian livestock industry develops, breeders of Brahman cattle have been using animal breeding tools to obtain better productive and qualitative results. The study of reproductive traits in beef cattle aims to identify sexually precocious animals that have reproductive longevity. However, most of these traits present low heritability estimates, which indicates that the response to selection would be slow. Thus, the objectives of this study were to estimate genetic parameters of productive, reproductive and carcass traits; evaluate the genetic trends over the years of selection; and the influence of inbreeding on phenotypes in Brahman cattle. The traits herein studied were: body weights measured at 120, 210, 365 and 450 days of age (P120, P210, P365, and P450); scrotal circumferences measured at 365 and 450 days of age (PE365 and PE450); age at first calving (IPP); gestation length (PG); stayability (STAY); rib eye area (AOL); subcutaneous fat thickness (EGS); and rump fat thickness (EGP8). The SAS statistical program was used to determine the significance of the fixed effects for the studied traits. The genetic parameters were estimated using WOMBAT and THRGIBBS1F90 software, under animal model, in single and two-trait analyses. The heritability estimate averages for P120, P210, P365, P450, PE365, PE450, IPP, PG, STAY, AOL, EGS, and EGP8 were equal to 0.19±0.03, 0.23±0.03, 0.23±0.03, 0.29±0.03, 0.26±0.05, 0.22±0.04, 0.10±0.03, 0.43±0.06, 0.14±0.04, 0.24±0.06, 0.17±0.06, and 0.27±0.07, respectively. Maternal heritability estimates for P120, P210, P365, P450, PE365, PE450 and PG were equal to 0.13 ± 0.03, 013 ± 0.03, 0.15 ± 0.03, 0.11 ± 0.03, 0.11 ± 0.02, 0.07 ± 0.02, and 0.07 ± 0.03, respectively. The proportion of the maternal permanent environment effect for P120 and P210 was equal to 0.11 ± 0.02. Genetic correlations ranged between -0.60 (STAY and EGS) and 0.97 (P365 and P450). Genetic trends were significant (p <0.05) and favorable for all traits, except for IPP. There was a significant effect (p <0.05) of inbreeding in relation to the phenotypes adjusted for the fixed effects of P120, P210, P365, P450, and IPP. Based on the genetic trends, it was possible to verify that the selection conducted on the studied traits has been efficient and resulted in favorable annual genetic gains. Reproductive traits of high economic importance, difficult to measure and with low heritability, such as IPP and STAY, can be indirectly assisted by the selection in PE365, considering their moderate heritability and favorable genetic correlations with these traits. The emphasis applied in the selection of body weight traits may indirectly assist AOL. Although not included in the selection index, EGS and EGP8 have responded to the selection due to the use of breeders with high genetic value for these traits.
  • FRANCISCO NAYSSON DE SOUSA SANTOS
  • TOTAL MIXED RATIONS BASED ON FORAGE CACTUS ON THE FERMENTATION PROFILE, TAXONOMIC DIVERSITY, PRODUCTION, MILK COMPOSITION AND ECONOMIC EVALUATION OF LACTATING GOATS
  • Date: Feb 8, 2021
  • Time: 13:00
  • Show Summary
  • The first aimed to evaluate the productive and morphometric characteristics of forage cactus and gliricídia intercropped at different spacings. The experimental design was in randomized blocks with 4 treatments and 6 replications. The treatments consisted of four spacings between rows, in the planting of gliricídia: E1 (1.0 m), E2 (1.50 m), E3 (2.0 m) and E4 (3.0 m), with 2, 30 m between plants. In the palm, the height, width, total number of cladodes in the plant, length of cladodes, cladode width, total cladode area were analyzed, green and dry matter production, rain use efficiency. There was a significant difference only for plant height and plant width, with increasing linear effect (P<0.05), for forage cactus during two evaluation cycles. The gliricídia in the first year of intercropping, the variables, planar width, number of leaves, weight of branches, green matter and plant dry matter presented differences (P<0.05) in the spacings. The gliricídia can be used in intercropping with the forage cactus at a spacing of 1.0 m since, in addition to not compromising the performance and production of green matter of the two crops, it increases the efficiency of the use of the area. In the second and third experimental trial, the objective was to evaluate the effects of forage cactus levels in complete feed silages on the fermentation profile, microbial populations, aerobic stability, taxonomic diversity, silage losses and in situ degradability of dry matter. The design used was completely randomized, in a 4x4 factorial arrangement with six replications, four rations with different proportions of forage cactus (15; 30; 45 and 60% based on natural matter) and four silo opening periods (0, 15, 30 and 60 days of fermentation). An interaction effect (P<0.05) was observed between the rations and the opening time for mold and yeast population. A significant difference was observed for the richness and diversity index (P<0.05) (Chao1 and Shannon test). The most abundant bacterial phyla were Proteobacteria and Firmicutes and the genera Lactobacillus and Weissella. Gas losses were minimal and without differences between treatments (P>0.05), remaining below 1% in all complete feed silages in the study. In addition, effluent losses were not significantly different (P>0.05) between the silages, with an average of 13.6 kg tol-1 being bserved. Forage cactus can be included in complete feed silages up to the 60% level, so it acted positively on the qualitative indicators of the silages, modulating the taxonomic communities and allowing the predominance of important groups for the preservation of the silage mass and greater recovery of matter dry. In the fourth experiment, the objective was to evaluate the effect of feeding dairy goats with total ration silage based on forage cactus and buffel grass hay on milk production and composition as well as on the economic indicators of milk production. For the experimental trial, eight crossbred, lactating multiparous dairy goats with 40 ± 2.0 kg of body weight were used. Distributed in a 4x4 double latin square, balanced according to the lactation period. There was a significant difference (P>0.05) regarding milk production and milk fat content. The profitability index was positive only for the diet with 30% inclusion of buffel grass hay, presenting a value of 1.6 R$, while the profitability rate was 60%. Diets with 30% of buffel grass hay promoted an increase in milk production and alterations in the percentage of fat. The simulation to produce 100 dairy goats per year provided revenues higher than production costs, resulting in positive economic indicators for all evaluated scenarios.
  • PEDRO HENRIQUE DE MELO GARCIA
  • Red propolis extract in the diet of confined sheep: morphometric changes of the digestive system
  • Date: Feb 2, 2021
  • Time: 09:00
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  • Red propolis stands out as a natural food additive, as it presents a potential source of bioactive compounds of great interest. The objective of this study was to evaluate the effect of the use of increasing levels of red propolis extract (RPE) in the diet of sheep confined on the morphometric parameters of rumen and intestine and histopathological parameters of liver and kidney. For this, 35 Santa Inês crossbreed lambs, non-castrated males, with a mean age of 6 months and initial body weight of 17.08 ± 2.36 kg, distributed in a completely randomized design, with five treatments (0, 7, 14, 21 and 28 mL of RPE/animal/day) and seven replicates, submitted to an experimental period of 68 days. The animal feed was the same for all treatments, and the concentrated bulky ratio of 60:40 was adopted, respectively, consisting of ground corn, soybean meal, mineral supplement and Tifton-85 hay. The feed was supplied twice a day, in the form of a complete mixture. The RPE doses were offered to the animals orally after feeding, with an interval of one hour, using a 10 mL continuous flow dosing gun. After 68 days of confinement, the animals were slaughtered and samples of rumen, small intestine, liver and kidney were collected for histological analysis. The data were submitted to analysis of variance and regression at the level of 5% probability. The variables dry matter intake (DMI), total weight gain (TWG) epithelium thickness (ET), keratinized portion of epithelium (KP), non-keratinized portion of epithelium (NKP), papilla height (PH), papilla width (PW), mucosa thickness (MT) and submucosa thickness (ST) exhibited quadratic curve behavior (P<0.05), except for the thickness of the muscle layer, duodenais goblet cell index and hepatic glycogen. The TWG showed an increase of 20.24% when compared to the animals of the 28 mL day-1 RPE treatment with the control group. It was verified that the use of RPE in the diet of sheep confined to the variables ET, NKP, PW, MT and ST had as maximum point estimated at levels 17 (174.38 μm), 14 (153.06 μm), 16 (429.36 μm), 33 (548.85 μm) and 9 (1382.52 μm) mL day-1 RPE, respectively, and obtained an estimated minimum point at levels 13 (0.65 kg of DM day-1), 11 (4.81 kg), 9 (19.99 μm) and 12 (1374.25 μm) mL day-1 RPE for the variables DMI, TWG, KP and PH, respectively. Based on the results obtained in the present study, it is recommended the level of 28 mL day-1 RPE in the diet of confined sheep, in order to provide greater absorptive areas in the digestive system, increase in total weight gain and does not cause histopathological changes in liver and kidneys.
  • GABRIEL FERREIRA DE LIMA CRUZ
  • CARCASS TRAITS AND MEAT QUALITY OF GOATS SUBJECTED TO AN INTERMITTENT WATER SUPPLY AND FED WITH CACTUS SILAGE
  • Date: Jan 29, 2021
  • Time: 08:00
  • Show Summary
  • The objective of this study was to evaluate the effect of using different proportions of forage cactus silage (SPF) (Opuntia fícus-indica Mill) and an intermittent water supply (OIA) for goats on carcass traits, meat quality, fatty acids profile and nutraceutical parameters. Thirty-six crossbreed with an initial average weight of 18.25 ± 7.23 kg and average of eight months were randomly assigned to a 3 × 3 factorial design comprising three addition ratios of SPF to the diet (0, 21 and 42% based on dry matter) and three water supply intervals (0, 24 and 48 hours) with four replicates, during 84 days of feedlot. There was no influence of diets, water supply and interaction between factors on biometric and morphometric measures, commercial cuts and tissue composition of the leg. There was a reduction in the weight of the full gastrointestinal tract and an increase in carcass yields as SPF was included in the animals' diet. The OIA caused an increase in the proportion of buchada in the percentage of weight at slaughter and a reduction in the percentage of leg fat of the animals. The remaining non-constituents of the carcass, rib eye area, cooking losses and carcass weight were not influenced by SPF and OIA. The color indices (L*, a*, b* and chroma), pH and the physical-chemical characteristics of the meat were not influenced, except for the ash content that showed an increase in the percentage of the meat when the animals were fed with 42% SPF diet. There was interaction between factors for other leg tissues, cooking losses and shear force of the L. lumborum muscle. OIA resulted in a percentage increase in palmitic fatty acid in the lipid profile of the animals' meat, and a reduction in the performance of the elongase enzyme. The replacement of Tifton hay by SPF in the animals' diet resulted in influences on the anteiso-pentadecanoic, behenic, tricosylic, cis-vaccenic, octadecanoic, gadoleic, α-linolenic, arachidonic, docosahexaenoic and sum of ω-6 fatty acids. However, SPF, OIA and the interaction between these factors did not change the sum and ratio of saturated, monounsaturated and polyunsaturated fatty acids, as well as in the other nutraceutical parameters. Therefore, in situations of water restriction, both strategies, intermittent supply of up to 48 hours and diets with of up to 42% forage cactus silage, can be adopted in goat confinement, producing meat and carcasses with considerable nutritional quality and relatively healthy to consumers.
  • MARINA DE SOUSA HIPOLITO FERREIRA
  • PERFORMANCE AND HISTOLOGICAL EVALUATION OF THE GOATS DIGESTORY DEVICE SUBMITTED TO DIFFERENT BREASTFEEDING STRATEGIES
  • Advisor : ARIOSVALDO NUNES DE MEDEIROS
  • Date: Jan 28, 2021
  • Time: 14:00
  • Show Summary
  • The objective of this work was to evaluate the performance of goats submitted to lactation strategies characterized by different amounts and periods of milk supply, considering the consumption of solid foods and their histological changes in the digestive system. Eighty crossbred Saanen × Alpine-American animals were used, 40 females and 600 40 whole males, distributed in a completely randomized design in a factorial scheme (5 601 × 2), considering as factors five breastfeeding systems and two sexes (8 males and 8 602 females) constituted according to the quantity and frequency of the milk offered, 603 comprising two stages of breastfeeding. In the first phase, the animals received: T1 = 1.2 604 kg milk / animal / day (morning / afternoon); T2 = 1.2 kg milk / animal / day (morning / 605 afternoon); T3 = 1.2 kg milk / animal / day (morning / afternoon); T4 = 1.0 kg milk / 606 animal / day (morning / afternoon); T5 = 1.0 kg milk / animal / day (morning / afternoon). 607 For the second phase: T1 = 1.0 kg milk / animal / day (morning / afternoon); T2 = 1.0 kg 608 milk / animal / day (morning); T3 = 1.0 kg milk / animal / day (afternoon); T4 = 0.8 kg 609 milk / animal / day (morning); T5 = 0.8 kg milk / animal / day (afternoon). The 610 breastfeeding strategies evaluated on performance verified the effect of sex (P <0.05), 611 where male goats had a higher weight than females from the 7th day of life until weaning. 612 The consumption of DM in% CP, MM, EE and PB were not influenced (P> 0.05) by the 613 treatment, sex or the interaction between them. However, there was a sex effect (P <0.05) 614 for the consumption of DM, CNF and CHOT, in which the male goats in this study had 615 higher consumption averages in relation to the females, showing higher averages for the 616 kids submitted to the T5 (0.8 / afternoon). The apparent digestibility coefficients were not 617 influenced (P> 0.05) by the different experimental feedings. In ruminal 618 histomorphometry, there was a significant effect on epithelial thickness and papilla width 619 (P = 0.01), with the highest values observed at T1 (0.5 morning / afternoon) in relation to 620 the other treatments. The thickness of the muscle layer was significant (P = 0.02) for 621 animals submitted to T4 (0.8 / morning), verifying the interaction effect for quantity (P = 622 0.036). There was also a difference between the treatments for the variable height of 623 papilla (P = 0.01) and absorption area (P = 0.02) in the animals that received T5 (0.8 / 624 afternoon), they showed interaction (P <0.05) between the quantities of milk offered. In 625 the histomorphometric parameters of the intestine, the breastfeeding strategies influenced 626 (P <0.05) in the villus width and submucosa thickness, in which higher means for T4 were observed, presenting an interaction effect (P <0.05) for frequency. There was a higher 628 index of goblet cells for animals fed T2 (1.0 / afternoon). T1 animals (1.0 / morning), 629 showed a higher (P <0.05) hepatic glycogen stock. The different breastfeeding systems 630 did not influence the N-consumed, N-feces, N-urine, N-balance and N-retained (P> 0.0) 631 The supply of more milk (1.0 kg), in the morning shift provides better animal 632 performance, without altering the morphological characteristics of the digestive system. 633 However, the use of a smaller amount of milk (0.8 kg) in the afternoon shift, allows larger 634 areas of rumen absorption.
  • FRANCINILDA ALVES DE SOUSA
  • RUMINAL BIOHYDROGENATION AND MEAT QUALITY OF SHEEP FED DIETS CONTAINING DIFFERENT SOURCES OF CARBOHYDRATES AND RICH IN POLYUNSATURATED FATTY ACIDS
  • Advisor : ARIOSVALDO NUNES DE MEDEIROS
  • Date: Jan 20, 2021
  • Time: 09:00
  • Show Summary
  • The aim of this study was to evaluate the effect of ruminal biohydrogenation, and meat quality of sheep fed diets containing different sources of carbohydrates rich in polyunsaturated fatty acids. Samples of ruminal, abomasal, and meat content of thirty male castrated lambs (20.5 ± 7.6 kg), with no defined racial pattern, were distributed in a completely randomized design under three experimental diets: High NFC diet (high proportion of non-fibrous carbohydrates); High NFC + spineless cactus diet (high proportion of non-fibrous carbohydrates and spineless cactus) and Low NFC diet (low proportion of non-fibrous carbohydrates), in a 60-day experimental period. The ruminal pH and short-chain fatty acid concentrations (SCFA) did not differ (P > 0.05) between diets. High NFC + Palm diet showed lower (P < 0.05) ruminal ammonia production, compared to the others. The High NFC + Palm diet showed the highest concentration (P < 0.05) of the total fatty acid (FA), AGC18, saturated fatty acids (SFA), 16: 0, 18: 0, c9-18: 1, c9, t11-CLA, and branched-chain AG (BCFA) for rumen content. In the abomasal content, the total concentrations of the biohydrogenation (BI), monounsaturated (MUFA) and trans-MUFA intermediates were significantly (P < 0.05) affected when using the spineless cactus in the High NFC diet. The Low NFC diet showed a higher estimate of biohydrogenation for c9-18:1, and a lower estimate of biohydrogenation for 18:3n-3 in the rumen and abomasum, respectively. The moisture and fat content of the meat were not affected (P> 0.05) by the types of diets offered to the animals. The physical characteristics of the meat, none of the variables evaluated were affected by the type of diet. There was no significant difference (P> 0.05) in the meat's antioxidant activity. The profile of single-chain saturated fatty acids in meat was not affected by the type of diet offered (P> 0.05). As for the saturated branched-chain fatty acids, we can see that the fatty acids, Iso-pentadecanoic (C15: 0 iso) and Iso-heptadecanoic (C17: 0 iso), were significantly higher in the meat of animals fed a Low NFC compared to animals fed a High NFC diet. High proportions of non-fibrous carbohydrates, regardless of the source, in diets rich in polyunsaturated fatty acids, promote improvements in the composition of sheep meat.